MLK3 Antibody (YA3237)(PBS only)

Cat. No.: HY-P83492A: Data Sheet User Guide for Antibodies
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MLK3 Antibody (YA3237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLK3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MLK3 Antibody (YA3237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLK3.

Host

Rabbit

Molecular Weight

Predicted band size: 93 kDa;

Observed band size: 93 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q16584

Gene ID

4296 [NCBI]

Immunogen

A synthetic peptide of human MLK3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_specificity:Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and human lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain

Isoforms & Post-Translational Modification：Q16584 has 2 isomers: Q16584-1: 92688 Da (predicted); Q16584-2: 65344 Da (predicted).
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42

Subunit：Homodimer; undergoes dimerization during activation (PubMed:9829970). Interacts with MAP2K7/MKK7 (PubMed:9003778).

Research Field

Signal Transduction

Synonyms

MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase

Documentation

User Guide for Antibodies (1077 KB)