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HARS Antibody (YA978)

(Synonyms: HRS; CMT2W; USH3B)
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Based on 1 Customer Validation

HARS Antibody (YA978) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HARS.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HARS Antibody (YA978)
  • HARS Antibody (YA978) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

HARS Antibody (YA978) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HARS.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database

Entrez Gene: 3035 Human

SwissProt: P12081 Human

Immunogen

A synthetic peptide of human HARS

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103350

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HARS Antibody (YA978)
    Western blot analysis of extracts from TF-1(lane 2(20ug) , U937(lane 3(20ug) and Jurkat(lane 4(20ug) using HARS Antibody (HY-P81278B) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    HARS catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:brain, heart, liver and kidneys

  • Isoforms & Post-Translational Modification

    P12081 has 4 isomers: P12081-1: 57411 Da (predicted); P12081-2: 52720 Da (predicted); P12081-3: 50156 Da (predicted); P12081-4: 54847 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P12081

  • Gene ID
    3035 [NCBI]
  • Synonyms

    HRS; CMT2W; USH3B

  • Research Field

    Immunology

HARS Antibody (YA978) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from TF-1(lane 2(20ug) , U937(lane 3(20ug) and Jurkat(lane 4(20ug) using HARS Antibody (HY-P81278B) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HARS Antibody (YA978) Product Image
    1/16
  • Experimental Validation Results for HARS Antibody (YA978)
  • HARS Antibody (YA978) Product Image
  • Experimental Validation Results for HARS Antibody (YA978)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from TF-1(lane 2(20ug) , U937(lane 3(20ug) and Jurkat(lane 4(20ug) using HARS Antibody (HY-P81278B) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HARS Antibody (YA978)
  • HARS Antibody (YA978) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from TF-1(lane 2(20ug) , U937(lane 3(20ug) and Jurkat(lane 4(20ug) using HARS Antibody (HY-P81278B) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HARS Antibody (YA978) Product Image WB