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Tryptophan 5 Hydroxylase 1 Antibody (YA2749)

(Synonyms: TPH1; MGC119994; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan Hydroxylase)
Customer Review

Based on 1 Customer Validation

Tryptophan 5 Hydroxylase 1 Antibody (YA2749) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tryptophan 5 Hydroxylase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

Tryptophan 5 Hydroxylase 1 Antibody (YA2749) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tryptophan 5 Hydroxylase 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TPH1 aa380-444.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105122

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    Western blot analysis of extracts from HL-60(lane 2(20ug) , THP-1(lane 3(20ug) and MCF-7(lane 4(20ug) using Tryptophan 5 Hydroxylase 1 Antibody (HY-P83004) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Tryptophan 5 Hydroxylase 1 oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis

  • Expression


    Tissue_specificity:Seems to be less widely expressed than isoform 1

  • Isoforms & Post-Translational Modification

    P17752 has 2 isomers: P17752-1: 50985 Da (predicted); P17752-2: 53394 Da (predicted).
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitinated is triggered by phosphorylation;Phosphorylated; triggering degradation by the proteasome

  • Subunit

    Homotetramer (By similarity). Interacts with DNAJC12 (By similarity)

  • SwissProt ID

    P17752

  • Gene ID
    7166 [NCBI]
  • Synonyms

    TPH1; MGC119994; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan Hydroxylase

  • Research Field

    Signal Transduction

Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) , THP-1(lane 3(20ug) and MCF-7(lane 4(20ug) using Tryptophan 5 Hydroxylase 1 Antibody (HY-P83004) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
    1/16
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) , THP-1(lane 3(20ug) and MCF-7(lane 4(20ug) using Tryptophan 5 Hydroxylase 1 Antibody (HY-P83004) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Experimental Validation Results for Tryptophan 5 Hydroxylase 1 Antibody (YA2749)
  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL-60(lane 2(20ug) , THP-1(lane 3(20ug) and MCF-7(lane 4(20ug) using Tryptophan 5 Hydroxylase 1 Antibody (HY-P83004) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tryptophan 5 Hydroxylase 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image WB
  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image IHC-P
  • Tryptophan 5 Hydroxylase 1 Antibody (YA2749) Product Image IHC-P