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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. ATF7 Antibody (YA2651)

ATF7 Antibody (YA2651) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF7.

For research use only. We do not sell to patients.

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20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

ATF7 Antibody (YA2651) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF7.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 52 kDa;
Observed band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human ATF7 aa2-49/483.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed:29490055). In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes (By similarity). Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation (By similarity). In response to TNF-alpha, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening (PubMed:29490055). Also plays a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair (By similarity); Acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter; Acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation
Subcellular Localization：Nucleus; Nucleus, nucleoplasm; Chromosome, telomere; Cytoplasm
Expression：
Tissue_specificity:Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta; Strongly expressed in skeletal muscle. Also expressed at lower levels in heart and lung
Isoforms & Post-Translational Modification：P17544 has 6 isomers: P17544-6: 51757 Da (predicted); P17544-1: 52967 Da (predicted); P17544-2: 49650 Da (predicted); P17544-3: 50860 Da (predicted); P17544-4: 33681 Da (predicted); P17544-5: 13278 Da (predicted).
On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity;Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase;On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity (PubMed:18950637). Social isolation stress as well as TNF-alpha also induce the phosphorylation of ATF7 (PubMed:29490055). Phosphorylated in proliferating colonic and small intestinal epithelial cells (By similarity)
Subunit：Homodimer; binds DNA as homodimer. Heterodimer; heterodimerizes with other members of ATF family and with JUN family members.
Database

Entrez Gene: 11016 Human ; 223922 Mouse ; 315333 Rat

SwissProt: P17544 Human ; Q8R0S1 Mouse ;

OMIM: 606371 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
ATF7; ATFA; Cyclic AMP-dependent transcription factor ATF-7; cAMP-dependent transcription factor ATF-7; Activating transcription factor 7; Transcription factor ATF-A
Documentation
User Guide for Antibodies (1077 KB)

ATF7 Antibody (YA2651) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ATF7 Antibody (YA2651)
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HY-P82906
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