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LMAN1 Antibody (YA1427)

(Synonyms: ERGIC5; F5F8D; FMFD1; Gp58; Lman1; MCFD1; MR60)
Customer Review

Based on 1 Customer Validation

LMAN1 Antibody (YA1427) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMAN1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC ICC
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • LMAN1 Antibody (YA1427) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

LMAN1 Antibody (YA1427) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMAN1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human LMAN1 aa1-70/510.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103790

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and JAR (lane 4, 15 μg) using LMAN1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunocytochemistry analysis of Hela cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    Immunocytochemistry analysis of PC-3 cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    LMAN1 is a Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins

  • Subcellular Localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Ubiquitous

  • Subunit

    Exists both as a covalent disulfide-linked homohexamer, and a complex of three disulfide-linked dimers non-covalently kept together. Interacts with MCFD2. May interact with TMEM115. Interacts with RAB3GAP1 and RAB3GAP2 (PubMed:22337587). Interacts with UBXN6 (PubMed:22337587). Interacts with SERPINA1/alpha1-antitrypsin (PubMed:31142615). Interacts with BET1 (PubMed:34779586)

  • SwissProt ID

    P49257

  • Gene ID
    3998 [NCBI]
  • Synonyms

    ERGIC5; F5F8D; FMFD1; Gp58; Lman1; MCFD1; MR60

  • Research Field

    Signal Transduction

LMAN1 Antibody (YA1427) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and JAR (lane 4, 15 μg) using LMAN1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LMAN1 Antibody (YA1427) Product Image
    1/16
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • LMAN1 Antibody (YA1427) Product Image
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and JAR (lane 4, 15 μg) using LMAN1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • Experimental Validation Results for LMAN1 Antibody (YA1427)
  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and JAR (lane 4, 15 μg) using LMAN1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using LMAN1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81682, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Lung Adenocarcinom tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma tissue using LMAN1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81682, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • LMAN1 Antibody (YA1427) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LMAN1 Antibody (YA1427) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling LMAN1 with LMAN1 Antibody (HY-P81682) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LMAN1 Antibody (HY-P81682) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LMAN1 Antibody (YA1427) Product Image WB
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image IHC-P
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image mIHC
  • LMAN1 Antibody (YA1427) Product Image ICC
  • LMAN1 Antibody (YA1427) Product Image ICC