CLK2 Antibody
(Synonyms: Dual specificity protein kinase CLK2, CDC-like kinase 2, CLK2)CLK2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CLK2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Monkey
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
CLK2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CLK2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Monkey
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 60 kDa
Synthetic peptide corresponding to the N-term region of human CLK2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CLK2 is a Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Endothelial cells. Expressed in androgen-dependent prostate cancer cells. -
Isoforms & Post-Translational Modification
CLK2 has 3 isoforms, P49760-1: amino acid length is 499, molecular weight is 60090 Da (predicted); P49760-2: amino acid length is 139, molecular weight is 17569 Da (predicted); P49760-3: amino acid length is 498, molecular weight is 59962 Da (predicted).可在所有三种类型的残基上发生自磷酸化。电离辐射或胰岛素刺激下，AKT1 可介导其 Ser-34 和 Thr-127 位点磷酸化。磷酸化在低剂量辐射后的细胞增殖中发挥关键作用，并可防止高剂量辐射后的细胞死亡。PKB/AKT2 介导的 Thr-344 位点磷酸化可诱导其激酶活性，这是维持其稳定性的必要条件。Ser-142 的磷酸化状态会影响其核内亚定位；抑制 Ser-142 磷酸化会导致其在核散斑中积累CLK2 存在 3 个异构体，P49760-1：氨基酸个数为 499 个，分子量为 60090 Da (预测)；P49760-2：氨基酸个数为 139 个，分子量为 17569 Da (预测)；P49760-3：氨基酸个数为 498 个，分子量为 59962 Da (预测)。Autophosphorylates on all three types of residues. Phosphorylation on Ser-34 and Thr-127 by AKT1 is induced by ionizing radiation or insulin. Phosphorylation plays a critical role in cell proliferation following low dose radiation and prevents cell death following high dose radiation. Phosphorylation at Thr-344 by PKB/AKT2 induces its kinase activity which is required for its stability. The phosphorylation status at Ser-142 influences its subnuclear localization; inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle
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Subunit
Interacts with RBMX.
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SwissProt ID
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Synonyms
Dual specificity protein kinase CLK2, CDC-like kinase 2, CLK2
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