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TIRAP Antibody (YA2589)

(Synonyms: MAL; Wyatt)
Customer Review

Based on 1 Customer Validation

TIRAP Antibody (YA2589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIRAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TIRAP Antibody (YA2589)
  • TIRAP Antibody (YA2589) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TIRAP Antibody (YA2589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIRAP.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 24 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TIRAP aa1-30.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104937

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIRAP Antibody (YA2589)
    Western blot analysis of extracts from HUVEC (lane 2(20μg)), THP-1+TPA (lane 3(20μg)), 293T (lane 4(20μg)),and Hela (lane 5(20μg)) using TIRAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    TIRAP is an Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6)

  • Subcellular Localization

    Cytoplasm; Cell membrane; Membrane

  • Expression


    Tissue_specificity:Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain

  • Isoforms & Post-Translational Modification

    P58753 has 3 isomers: P58753-1: 23883 Da (predicted); P58753-2: 25323 Da (predicted); P58753-3: 27948 Da (predicted).
    Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK;Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation

  • Subunit

    Homodimer (PubMed:17322885). Also forms heterodimers with MYD88 (PubMed:17322885). May interact with PIK3AP1 (By similarity). Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with BMX and TBK1. Interacts with EIF2AK2. Does not interact with IRAK1, nor TLR9. Interacts with TLR2 (PubMed:17322885). Interacts with RAGE/AGER (PubMed:21829704)

  • SwissProt ID

    P58753

  • Gene ID
    114609 [NCBI]
  • Synonyms

    MAL; Wyatt

  • Research Field

    Immunology

TIRAP Antibody (YA2589) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg)), THP-1+TPA (lane 3(20μg)), 293T (lane 4(20μg)),and Hela (lane 5(20μg)) using TIRAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TIRAP Antibody (YA2589) Product Image
    1/16
  • Experimental Validation Results for TIRAP Antibody (YA2589)
  • TIRAP Antibody (YA2589) Product Image
  • Experimental Validation Results for TIRAP Antibody (YA2589)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg)), THP-1+TPA (lane 3(20μg)), 293T (lane 4(20μg)),and Hela (lane 5(20μg)) using TIRAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIRAP Antibody (YA2589)
  • TIRAP Antibody (YA2589) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVEC (lane 2(20μg)), THP-1+TPA (lane 3(20μg)), 293T (lane 4(20μg)),and Hela (lane 5(20μg)) using TIRAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TIRAP Antibody (YA2589) Product Image WB