TIRAP Antibody (YA2589)

Cat. No.: HY-P82844: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TIRAP Antibody (YA2589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIRAP.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TIRAP Antibody (YA2589) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

TIRAP Antibody (YA2589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIRAP.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 24 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P58753

Gene ID

114609 [NCBI]

Immunogen

A synthesized peptide derived from human TIRAP aa1-30.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HUVEC (lane 2(20μg)), THP-1+TPA (lane 3(20μg)), 293T (lane 4(20μg)),and Hela (lane 5(20μg)) using TIRAP Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6)

Subcellular Localization：Cytoplasm; Cell membrane; Membrane

Expression：
Tissue_specificity:Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain

Isoforms & Post-Translational Modification：P58753 has 3 isomers: P58753-1: 23883 Da (predicted); P58753-2: 25323 Da (predicted); P58753-3: 27948 Da (predicted).
Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK;Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation

Subunit：Homodimer (PubMed:17322885). Also forms heterodimers with MYD88 (PubMed:17322885). May interact with PIK3AP1 (By similarity). Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with BMX and TBK1. Interacts with EIF2AK2. Does not interact with IRAK1, nor TLR9. Interacts with TLR2 (PubMed:17322885). Interacts with RAGE/AGER (PubMed:21829704)

RRID

AB_3104937

Database

Entrez Gene: 114609 Human ; 117149 Mouse ; 680127 Rat

SwissProt: P58753 Human ; Q99JY1 Mouse ;

OMIM: 606252 Human

Research Field

Immunology

Synonyms

MAL; Wyatt

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

TIRAP Antibody (YA2589) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TIRAP Antibody (YA2589)

TIRAP Antibody (YA2589) Featured Recommendations:

Product Detail

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Background

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TIRAP Antibody (YA2589) Related Classifications

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