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FABP4 Antibody (YA7593)

Customer Review

Based on 1 Customer Validation

FABP4 Antibody (YA7593) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to FABP4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • FABP4 Antibody (YA7593) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:1000-5000 1:200-1000 1:5000-20000

Product Details

Description

FABP4 Antibody (YA7593) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to FABP4.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FABP4 Antibody (YA7593)
    Western blot analysis of extracts from Mouse white adipose (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Rat white adipose (lane 3(20μg)) and Rat heart (lane 4(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FABP4 Antibody (YA7593)
    Western blot analysis of extracts from Human breast (lane 1(20μg)) and Human breast cancer (lane 2(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    FABP4 is a Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Isoforms & Post-Translational Modification

    P15090: 132 amino acids, molecular weight 14719 Da.

  • Subunit

    Monomer (PubMed:15357969, PubMed:17077479)

  • SwissProt ID

    P15090

FABP4 Antibody (YA7593) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse white adipose (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Rat white adipose (lane 3(20μg)) and Rat heart (lane 4(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Human breast (lane 1(20μg)) and Human breast cancer (lane 2(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • FABP4 Antibody (YA7593) Product Image
    1/16
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • FABP4 Antibody (YA7593) Product Image
  • Experimental Validation Results for FABP4 Antibody (YA7593)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse white adipose (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Rat white adipose (lane 3(20μg)) and Rat heart (lane 4(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FABP4 Antibody (YA7593)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Human breast (lane 1(20μg)) and Human breast cancer (lane 2(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • Experimental Validation Results for FABP4 Antibody (YA7593)
  • FABP4 Antibody (YA7593) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse white adipose (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Rat white adipose (lane 3(20μg)) and Rat heart (lane 4(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • FABP4 Antibody (YA7593) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Human breast (lane 1(20μg)) and Human breast cancer (lane 2(20μg)) using FABP4 Antibody (HY-P87908) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • FABP4 Antibody (YA7593) Product Image WB
  • FABP4 Antibody (YA7593) Product Image WB