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EYA2 Antibody (YA2905)

(Synonyms: EAB1)
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Based on 1 Customer Validation

EYA2 Antibody (YA2905) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EYA2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EYA2 Antibody (YA2905)
  • EYA2 Antibody (YA2905) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

EYA2 Antibody (YA2905) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EYA2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 59 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Species Reactivity Database

Entrez Gene: 2139 Human

SwissProt: O00167 Human

Immunogen

A synthetic peptide of human EYA2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105275

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EYA2 Antibody (YA2905)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), A549 (lane 3, 15 μg), SK-BR-3 (lane 4, 15 μg), and BxPC-3 (lane 5, 15 μg) using EYA2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    EYA2 functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1[1][2][3].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart

  • Isoforms & Post-Translational Modification

    O00167 has 3 isomers: O00167-1: 59232 Da (predicted); O00167-2: 56688 Da (predicted); O00167-3: 50533 Da (predicted).

  • Subunit

    Interacts with DACH2 and SIX1, and probably with SIX2, SIX4 and SIX5. Interacts with CAPN8 (By similarity). Interacts with GNAZ and GNAI2; this precludes interaction with SIX1

  • SwissProt ID

    O00167

  • Gene ID
    2139 [NCBI]
  • Synonyms

    EAB1

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Fougerousse F, et al. Six and Eya expression during human somitogenesis and MyoD gene family activation. J Muscle Res Cell Motil. 2002;23(3):255-64. [Content Brief]

[2]. Patrick AN, et al. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nat Struct Mol Biol. 2013 Apr;20(4):447-53. [Content Brief]

[3]. Krishnan N, et al. Dephosphorylation of the C-terminal tyrosyl residue of the DNA damage-related histone H2A.X is mediated by the protein phosphatase eyes absent. J Biol Chem. 2009 Jun 12;284(24):16066-16070. [Content Brief]

EYA2 Antibody (YA2905) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), A549 (lane 3, 15 μg), SK-BR-3 (lane 4, 15 μg), and BxPC-3 (lane 5, 15 μg) using EYA2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • EYA2 Antibody (YA2905) Product Image
    1/16
  • Experimental Validation Results for EYA2 Antibody (YA2905)
  • EYA2 Antibody (YA2905) Product Image
  • Experimental Validation Results for EYA2 Antibody (YA2905)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), A549 (lane 3, 15 μg), SK-BR-3 (lane 4, 15 μg), and BxPC-3 (lane 5, 15 μg) using EYA2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for EYA2 Antibody (YA2905)
  • EYA2 Antibody (YA2905) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), A549 (lane 3, 15 μg), SK-BR-3 (lane 4, 15 μg), and BxPC-3 (lane 5, 15 μg) using EYA2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • EYA2 Antibody (YA2905) Product Image WB