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TBR1 Antibody (YA6335)

Customer Review

Based on 1 Customer Validation

TBR1 Antibody (YA6335) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBR1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • TBR1 Antibody (YA6335) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1000 1:1000-5000 1:200-1000 1:5000-20000 1:50-200

Product Details

Description

TBR1 Antibody (YA6335) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBR1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 74 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 74 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719194

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Western blot analysis of extracts from Raji(lane 2(20ug) , U937(lane 3(20ug) and 3T3-L1(lane 4(20ug) , THP-1(lane 5(20ug) using TBR1 Antibody(HY-P86643) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using TBR1 Antibody (HY-P86643, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    TBR1 transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZF2, it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons[1][2].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:brain

  • Isoforms & Post-Translational Modification

    Q16650 has 2 isomers: Q16650-1: 74053 Da (predicted); Q16650-2: 42332 Da (predicted).

  • Subunit

    Homodimer (PubMed:25232744). Part of a complex containing CASK, TBR1 and TSPYL2; may modulate gene expression in response to neuronal synaptic activity (By similarity). Interacts with FOXP2 (PubMed:25232744, PubMed:30250039). Interacts with FOXP1 (PubMed:30250039). Interacts with BCL11A (PubMed:30250039)

  • SwissProt ID

    Q16650

  • Gene ID
    10716 [NCBI]

TBR1 Antibody (YA6335) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) , U937(lane 3(20ug) and 3T3-L1(lane 4(20ug) , THP-1(lane 5(20ug) using TBR1 Antibody(HY-P86643) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using TBR1 Antibody (HY-P86643, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image
    1/16
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • TBR1 Antibody (YA6335) Product Image
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) , U937(lane 3(20ug) and 3T3-L1(lane 4(20ug) , THP-1(lane 5(20ug) using TBR1 Antibody(HY-P86643) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using TBR1 Antibody (HY-P86643, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • Experimental Validation Results for TBR1 Antibody (YA6335)
  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji(lane 2(20ug) , U937(lane 3(20ug) and 3T3-L1(lane 4(20ug) , THP-1(lane 5(20ug) using TBR1 Antibody(HY-P86643) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using TBR1 Antibody (HY-P86643, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using TBR1 Antibody (HY-P86643, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBR1 Antibody (YA6335) Product Image WB
  • TBR1 Antibody (YA6335) Product Image IHC-P
  • TBR1 Antibody (YA6335) Product Image IHC-P
  • TBR1 Antibody (YA6335) Product Image IHC-P
  • TBR1 Antibody (YA6335) Product Image IHC-P
  • TBR1 Antibody (YA6335) Product Image IHC-P