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Peroxiredoxin 6 Antibody (YA3154)

(Synonyms: Peroxiredoxin-6; Antioxidant protein 2; Liver 2D page spot 40; NSGPx; PRDX6; AOP2; KIAA)
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Based on 1 Customer Validation

Peroxiredoxin 6 Antibody (YA3154) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Peroxiredoxin 6 Antibody (YA3154)
  • Peroxiredoxin 6 Antibody (YA3154) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Peroxiredoxin 6 Antibody (YA3154) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 9588 Human

SwissProt: P30041 Human

Immunogen

A synthetic peptide of human Peroxiredoxin 6 aa204-216.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105520

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Peroxiredoxin 6 Antibody (YA3154)
    Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug) and A549(lane 4(20ug) using Peroxiredoxin 6 Antibody (HY-P83409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Peroxiredoxin 6 is a TH2O2-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis. Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate[1][2][3].

  • Subcellular Localization

    Cytoplasm; Lysosome

  • Subunit

    Homodimer (PubMed:27353378, PubMed:9587003). Interacts with APEX1 (PubMed:19188445). Interacts with STH (PubMed:16186110). May interact with FAM168B (PubMed:20716133).

  • SwissProt ID

    P30041

  • Gene ID
    9588 [NCBI]
  • Synonyms

    Peroxiredoxin-6; Antioxidant protein 2; Liver 2D page spot 40; NSGPx; PRDX6; AOP2; KIAA

  • Research Field

    Signal Transduction

Peroxiredoxin 6 Antibody (YA3154) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug) and A549(lane 4(20ug) using Peroxiredoxin 6 Antibody (HY-P83409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Peroxiredoxin 6 Antibody (YA3154) Product Image
    1/16
  • Experimental Validation Results for Peroxiredoxin 6 Antibody (YA3154)
  • Peroxiredoxin 6 Antibody (YA3154) Product Image
  • Experimental Validation Results for Peroxiredoxin 6 Antibody (YA3154)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug) and A549(lane 4(20ug) using Peroxiredoxin 6 Antibody (HY-P83409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Peroxiredoxin 6 Antibody (YA3154)
  • Peroxiredoxin 6 Antibody (YA3154) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug) and A549(lane 4(20ug) using Peroxiredoxin 6 Antibody (HY-P83409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Peroxiredoxin 6 Antibody (YA3154) Product Image WB