PHAPI2/APRIL Antibody (YA1610)(PBS only)

Cat. No.: HY-P81865A: Data Sheet User Guide for Antibodies
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PHAPI2/APRIL Antibody (YA1610) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PHAPI2/APRIL Antibody (YA1610) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

Host

Rabbit

Molecular Weight

Predicted band size: 29 kDa;

Observed band size: 29 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q92688

Gene ID

10541 [NCBI]

Immunogen

A synthetic peptide of human PHAPI2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed:18039846, PubMed:20015864). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed:20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed:18039846, PubMed:20538007). Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed:17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity); (Microbial infection) Plays an essential role in influenza A and B viral genome replication (PubMed:31217244, PubMed:33045004). Also plays a role in foamy virus mRNA export from the nucleus to the cytoplasm (PubMed:21159877)

Subcellular Localization：Nucleus; Cytoplasm; Cytoplasm

Expression：
Tissue_specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta

Isoforms & Post-Translational Modification：Q92688 has 2 isomers: Q92688-1: 28788 Da (predicted); Q92688-2: 22277 Da (predicted).
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity);Directly cleaved by caspase-3/CASP3

Subunit：Interacts with histones H3 and H4 (PubMed:20538007). Interacts with KLF5; this interaction induces promoter region-specific histone incorporation and inhibition of histone acetylation by ANP32B (PubMed:18039846)

Research Field

Cell Biology

Synonyms

ANP32B; PAL31; PHAPI2; PHAPI2a; Ssp29

Documentation

User Guide for Antibodies (1077 KB)