2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Brk/PTK6 Antibody (YA4758)

Brk/PTK6 Antibody (YA4758)

製品番号: HY-P85066
User Guide for Antibodies Technical Support

Brk/PTK6 Antibody (YA4758) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Brk/PTK6.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Brk/PTK6 Antibody (YA4758) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Brk/PTK6.
Host

Mouse
Clonality

Monoclonal
分子量

Predicted band size: 52 kDa;

Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human Brk expressed in E. Coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
純度 affinity purified. Conjugation Non-conjugated
Modification Unmodified  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Phosphorylates the GTPase-activating protein ARAP1 following EGF stimulation which enhances EGFR signaling by delaying EGFR down-regulation (PubMed:20554524). Also associates with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways; Inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins
Subcellular Localization：Cytoplasm; Nucleus; Cell projection, ruffle; Membrane
Expression：
Tissue_specificity:Epithelial-specific expression. Extremely high expression levels in the colon, high levels in the small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissues, but expressed in a high proportion of breast and ovarian cancers. Overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas, and prostate cancers. Also found in melanocytes. Not expressed in the heart, brain, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. Isomer 2 is present in prostatic epithelial cell lines derived from normal prostate and prostate adenocarcinoma, as well as in various other cell lines.
Isoforms & Post-Translational Modification：Q13882 has 2 isomers: Q13882-1: 51834 Da (predicted); Q13882-2: 14465 Da (predicted).
Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity
Subunit：Interacts (via SH3 and SH2 domains) with KHDRBS1. Interacts (via SH3 and SH2 domains) with phosphorylated IRS4. Interacts with ADAM15. Interacts (via SH3 domain) with SFPQ. Interacts with EGFR and ERBB2.
Synonyms
PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Brk/PTK6 Antibody (YA4758) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Brk/PTK6 Antibody (YA4758)
製品番号:
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