GNAI2 Antibody (YA2944)(PBS only)

Cat. No.: HY-P83199A: Data Sheet User Guide for Antibodies
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GNAI2 Antibody (YA2944) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNAI2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GNAI2 Antibody (YA2944) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNAI2.

Host

Rabbit

Molecular Weight

Predicted band size: 40 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P04899

Gene ID

2771 [NCBI]

Immunogen

A synthetic peptide of human GNAI2 aa291-304.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division; Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cell membrane; Membrane; Lipid-anchor

Isoforms & Post-Translational Modification：P04899 has 6 isomers: P04899-1: 40451 Da (predicted); P04899-2: 38473 Da (predicted); P04899-3: 36465 Da (predicted); P04899-4: 41548 Da (predicted); P04899-5: 38737 Da (predicted); P04899-6: 34935 Da (predicted).
(Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA

Subunit：G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. In this context, interacts with GNB2 (PubMed:28219978). Interacts with GPSM1 (By similarity). Interacts with RGS12 and RGS14 (By similarity). Interacts with UNC5B (PubMed:12359238). Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity). Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif) (PubMed:26126266). Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (PubMed:19211784). Interacts with CXCR1 and CXCR2 (PubMed:8662698)

Research Field

Signal Transduction

Synonyms

guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 2; GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1

Documentation

User Guide for Antibodies (1077 KB)