Caldesmon Antibody (YA4187)(PBS only)

Cat. No.: HY-P84490A: Data Sheet User Guide for Antibodies
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Caldesmon Antibody (YA4187) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caldesmon.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Caldesmon Antibody (YA4187) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caldesmon.

Host

Mouse

Molecular Weight

Predicted band size: 93 kDa;

Observed band size: 70-75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q05682

Gene ID

800 [NCBI]

Immunogen

Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, myofibril; Cytoplasm, cytoskeleton, stress fiber

Expression：
Tissue_specificity:High molecular weight calmodulin (isoform 1) is primarily expressed in smooth muscle, while low molecular weight calmodulin (isoforms 2, 3, 4, and 5) are widely distributed in non-muscle tissues and cells. It is not expressed in skeletal muscle or the heart.

Isoforms & Post-Translational Modification：Q05682 has 6 isomers: Q05682-1: 93231 Da (predicted); Q05682-2: 65707 Da (predicted); Q05682-3: 64256 Da (predicted); Q05682-4: 62663 Da (predicted); Q05682-5: 61213 Da (predicted); Q05682-6: 65620 Da (predicted).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity)

Synonyms

CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22

Documentation

User Guide for Antibodies (1077 KB)