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Sprouty 4 Antibody (YA1780)

(Synonyms: Sprouty homolog 4; SPRY 4; Spry-4)
Customer Review

Based on 1 Customer Validation

Sprouty 4 Antibody (YA1780) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Sprouty 4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Sprouty 4 Antibody (YA1780) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50 1:50-1:100

Product Details

Description

Sprouty 4 Antibody (YA1780) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Sprouty 4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Sprouty 4 aa200-250/299.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), U251MG (lane 2, 15 μg), K562 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Sprouty 4/Spry-4 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    Western blot analysis of extracts from MCF-7 (lane 2(20μg), 3T3 (lane 3(20μg), C6 (lane 4(20μg) and K562 (lane 5(20μg) using Sprouty 4 Antibody (YA1780) (HY-P82035) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Sprouty 4 suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin[1][2][3].

  • Subcellular Localization

    Cytoplasm; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side

  • Isoforms & Post-Translational Modification

    Q9C004 has 2 isomers: Q9C004-1: 32541 Da (predicted); Q9C004-2: 11482 Da (predicted).

  • Subunit

    Interacts (via C-terminus) with TESK1 (via both C- and N-termini); the interaction inhibits TESK1 kinase activity (PubMed:12027893, PubMed:15584898). Interacts with RAF1 (PubMed:12717443). Interacts with CAV1 (via C-terminus) (By similarity)

  • SwissProt ID

    Q9C004

  • Gene ID
    81848 [NCBI]
  • Synonyms

    Sprouty homolog 4; SPRY 4; Spry-4

  • Research Field

    Signal Transduction

Sprouty 4 Antibody (YA1780) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), U251MG (lane 2, 15 μg), K562 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Sprouty 4/Spry-4 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), 3T3 (lane 3(20μg), C6 (lane 4(20μg) and K562 (lane 5(20μg) using Sprouty 4 Antibody (YA1780) (HY-P82035) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Sprouty 4 Antibody (YA1780) Product Image
    1/16
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Sprouty 4 Antibody (YA1780) Product Image
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), U251MG (lane 2, 15 μg), K562 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Sprouty 4/Spry-4 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), 3T3 (lane 3(20μg), C6 (lane 4(20μg) and K562 (lane 5(20μg) using Sprouty 4 Antibody (YA1780) (HY-P82035) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Experimental Validation Results for Sprouty 4 Antibody (YA1780)
  • Sprouty 4 Antibody (YA1780) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), U251MG (lane 2, 15 μg), K562 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Sprouty 4/Spry-4 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Sprouty 4 Antibody (YA1780) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2(20μg), 3T3 (lane 3(20μg), C6 (lane 4(20μg) and K562 (lane 5(20μg) using Sprouty 4 Antibody (YA1780) (HY-P82035) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Sprouty 4 Antibody (YA1780) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Sprouty 4 Antibody (YA1780) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Sprouty 4 Antibody(HY-P82035) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Sprouty 4 Antibody (HY-P82035) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Sprouty 4 Antibody (YA1780) Product Image WB
  • Sprouty 4 Antibody (YA1780) Product Image WB
  • Sprouty 4 Antibody (YA1780) Product Image ICC
  • Sprouty 4 Antibody (YA1780) Product Image ICC