VIMP Antibody (YA4458)

Cat. No.: HY-P84761: Data Sheet COA
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VIMP Antibody (YA4458) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VIMP.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

VIMP Antibody (YA4458) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VIMP.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BQE4

Gene ID

55829 [NCBI]

Immunogen

Purified recombinant fragment of human VIMP (AA: 1-187) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7 (lane 2(20μg), PANC-1 (lane 3(20μg), Jurkat (lane 4(20μg), HepG2 (lane 5(20μg) using VIMP Antibody（HY-P84761). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from MOLT4(lane 1(40μg) ), Raji(lane 2(40μg) ), HepG2(lane 3(40μg) ), Jurkat(lane 4(40μg) ), U251(lane 5(40μg) ), A431(lane 6(40μg) ), MCF-7(lane 7(40μg) ) and PANC-1(lane 8(40μg) )using VIMP antibody(31044P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasm

Subunit：Interacts with DERL1 and (via VIM motif) with VCP, suggesting that it forms a membrane complex with DERL1 that serves as a receptor for VCP. Also interacts with DERL2, DERL3 and SELENOK. The SELENOK-SELENOS complex interacts with VCP. Interacts with CCDC47 (By similarity)

RRID

AB_3718980

Synonyms

SELS; ADO15; SBBI8; SEPS1; AD-015

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)