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MFN1 Antibody (YA5815)

(Synonyms: Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1; )
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Based on 1 Customer Validation

MFN1 Antibody (YA5815) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MFN1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MFN1 Antibody (YA5815)
  • MFN1 Antibody (YA5815) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:2000-1:10000

Product Details

Description

MFN1 Antibody (YA5815) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MFN1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 84 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MFN1 Antibody (YA5815)
    Western blot analysis of extracts from K562 (lane2, 20μg), Hela (lane3, 20μg), PC-3 (lane4, 20μg) and A431 (lane5, 20μg) using MFN1 Antibody (HY-P86123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MFN1 is a Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity[1][2][3][4].

  • Subcellular Localization

    Mitochondrion outer membrane; Multi-pass membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Detected in kidney and heart (at protein level) (PubMed:12759376) . Ubiquitous (PubMed:11950885, PubMed:12759376) . Expressed at slightly higher level in kidney and heart (PubMed:12759376) . Isoform 2 may be overexpressed in some tumors, such as lung cancers (PubMed:11751411)

  • Isoforms & Post-Translational Modification

    Q8IWA4 has 3 isomers: Q8IWA4-1: 84160 Da (predicted); Q8IWA4-2: 41883 Da (predicted); Q8IWA4-3: 71401 Da (predicted).
    Ubiquitinated by non-degradative ubiquitin by PRKN (PubMed:23933751). Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCHF5 (PubMed:20103533). When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN (PubMed:23933751)

  • Subunit

    Homodimer, also in the absence of bound GTP (PubMed:27920125, PubMed:28114303). Forms higher oligomers in the presence of a transition state GTP analog (PubMed:28114303).

  • SwissProt ID

    Q8IWA4

  • Gene ID
    55669 [NCBI]
  • Synonyms

    Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1;

MFN1 Antibody (YA5815) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), Hela (lane3, 20μg), PC-3 (lane4, 20μg) and A431 (lane5, 20μg) using MFN1 Antibody (HY-P86123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • MFN1 Antibody (YA5815) Product Image
    1/16
  • Experimental Validation Results for MFN1 Antibody (YA5815)
  • MFN1 Antibody (YA5815) Product Image
  • Experimental Validation Results for MFN1 Antibody (YA5815)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), Hela (lane3, 20μg), PC-3 (lane4, 20μg) and A431 (lane5, 20μg) using MFN1 Antibody (HY-P86123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MFN1 Antibody (YA5815)
  • MFN1 Antibody (YA5815) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane2, 20μg), Hela (lane3, 20μg), PC-3 (lane4, 20μg) and A431 (lane5, 20μg) using MFN1 Antibody (HY-P86123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • MFN1 Antibody (YA5815) Product Image WB