MFN1 Antibody (YA5815)

Cat. No.: HY-P86123: Data Sheet COA
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MFN1 Antibody (YA5815) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MFN1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MFN1 Antibody (YA5815) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MFN1.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 84 kDa;

Observed band size: 84 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8IWA4

Gene ID

55669 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303)

Subcellular Localization：Mitochondrion outer membrane; Multi-pass membrane protein; Cytoplasm

Expression：
Tissue_specificity:Detected in kidney and heart (at protein level) (PubMed:12759376) . Ubiquitous (PubMed:11950885, PubMed:12759376) . Expressed at slightly higher level in kidney and heart (PubMed:12759376) . Isoform 2 may be overexpressed in some tumors, such as lung cancers (PubMed:11751411)

Isoforms & Post-Translational Modification：Q8IWA4 has 3 isomers: Q8IWA4-1: 84160 Da (predicted); Q8IWA4-2: 41883 Da (predicted); Q8IWA4-3: 71401 Da (predicted).
Ubiquitinated by non-degradative ubiquitin by PRKN (PubMed:23933751). Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCHF5 (PubMed:20103533). When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN (PubMed:23933751)

Subunit：Homodimer, also in the absence of bound GTP (PubMed:27920125, PubMed:28114303). Forms higher oligomers in the presence of a transition state GTP analog (PubMed:28114303).

Synonyms

Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)