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Phospho-PKC (Ser660) Antibody (YA1394)

(Synonyms: PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha)
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Phospho-PKC (Ser660) Antibody (YA1394) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PKC (Ser660).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Phospho-PKC (Ser660) Antibody (YA1394) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Phospho-PKC (Ser660) Antibody (YA1394) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PKC (Ser660).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78-85 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser660 of human PKC

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    PKC (Protein kinase C) is a family of protein kinase enzymes that are involved in controlling the function of otherproteins through the phosphorylation of hydroxyl groups of serine and threonine amino acid residues on these proteins. PKC enzymes in turn are activated by signals such as increases in the concentration of diacylglycerol (DAG) or calcium ions (Ca2+) . Hence PKC enzymes play important roles in several signal transduction cascades. The PKC family consists of 15 isozymes in humans: PKC-α (PRKCA), PKC-β1 (PRKCB), PKC-β2 (PRKCB), PKC-γ (PRKCG), PKC-δ (PRKCD), PKC-δ1 (PRKD1), PKC-δ2 (PRKD2), PKC-δ3 (PRKD3), PKC-ε (PRKCE), PKC-η (PRKCH), PKC-θ (PRKCQ), PKC-ι (PRKCI), PKC-ζ (PRKCZ), PK-N1 (PKN1), PK-N2 (PKN2), PK-N3 (PKN3) . PKC is involved in receptor desensitization, in modulating membrane structure events, in regulating transcription, in mediating immune responses, in regulating cell growth, and in learning and memory. These functions are achieved by PKC-mediated phosphorylation of other proteins.

  • Subcellular Localization

    P17252: Cytoplasm; Cell membrane; Peripheral membrane protein; Mitochondrion membrane; Peripheral membrane protein; Nucleus
    P05771: Cytoplasm; Nucleus; Membrane; Peripheral membrane protein
    Q05655: Cytoplasm; Cytoplasm, perinuclear region; Nucleus; Cell membrane; Peripheral membrane protein; Mitochondrion; Endomembrane system
    Q02156: Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Cytoplasm, perinuclear region; Nucleus
    P24723: Cytoplasm
    Q04759: Cytoplasm; Cell membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity: Q02156: Expressed in cumulus cells (at protein level)
    P24723: Most abundant in lung, less in heart and skin
    Q04759: Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets
    Induction: P24723: By stress conditions caused by amino acid starvation (at protein level)

  • Subunit

    P17252: Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and RACK1 (By similarity). Interacts with ADAP1/CENTA1 (PubMed:12893243). Interacts with CSPG4 (PubMed:15504744).
    P05771: Interacts with PDPK1/PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and AR
    Q05655: Interacts with PDPK1 (via N-terminal region) (PubMed:11781095). Interacts with RAD9A (PubMed:12628935). Interacts with CDCP1 (PubMed:15851033). Interacts with MUC1 (PubMed:11877440). Interacts with VASP (PubMed:16940418).
    Q02156: Forms a ternary complex with TRIM63 and RACK1/GN2BL1 (By similarity).
    P24723: Interacts with FYN (By similarity). Interacts with RALA (By similarity). Interacts with DGKQ (PubMed:15632189). Interacts with PRKCH upstream open reading frame 2; the interaction leads to inhibition of kinase activity (PubMed:34593629)
    Q04759: Part of a lipid raft complex composed at least of BCL10, CARD11, MALT1 and IKBKB (PubMed:16356855). Interacts with GLRX3 (via N-terminus) (PubMed:10636891). Interacts with ECT2 (PubMed:15254234).

  • SwissProt ID

    P17252 / P05771 / Q05655 / Q02156 / P24723 / Q04759

  • Gene ID
    5578 [NCBI]
  • Synonyms

    PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha

  • Research Field

    Signal Transduction

References

[1]. Lee MJ, et al. Time to HIV rebound after infusion of long-acting broadly neutralising antibodies 3BNC117-LS and 10-1074-LS and analytical treatment interruption (the RIO trial): a double-blind, randomised, placebo-controlled trial. Lancet HIV. 2026 May 27:S2352-3018(26)00059-7. [Content Brief]

[2]. Wolf S, et al. Selection for Evi1 activation in myelomonocytic leukemia induced by hyperactive signaling through wild-type NRas. Oncogene. 2013 Jun 20;32(25):3028-38. [Content Brief]

[3]. Ali SR, et al. Nerve Density and Neuronal Biomarkers in Cancer. Cancers (Basel). 2022 Oct 1;14(19):4817. [Content Brief]

[4]. Kim JY, et al. The induction of STAT1 gene by activating transcription factor 3 contributes to pancreatic beta-cell apoptosis and its dysfunction in streptozotocin-treated mice. Cell Signal. 2010 Nov;22(11):1669-80. [Content Brief]

[5]. Ciglenecki I, et al. Low sensitivity of the fourth-generation antigen/antibody HIV rapid diagnostic test Determine™ HIV Early Detect for detection of acute HIV infection at the point of care in rural Eswatini: a diagnostic accuracy study. J Int AIDS Soc. 2025 Jul;28(7):e26517. [Content Brief]

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Phospho-PKC (Ser660) Antibody (YA1394) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Phospho-PKC (Ser660) Antibody (YA1394) Product Image