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SIRT4 Antibody (YA6962)

(Synonyms: MGC130046 antibody; MGC130047 antibody; MGC57437 antibody; NAD dependent deacetylase sirtuin 4 antibody; NAD dependent protein deacetylase sirtuin 4 antibody; NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody; NAD-dependent protein deacetylase sirtuin-4 antibody; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial antibody; Regulatory protein SIR2 homolog 4 antibody; Silent mating type information regulation 2 homolog antibody)
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Based on 1 Customer Validation

SIRT4 Antibody (YA6962) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • SIRT4 Antibody (YA6962) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:200 1-2μg/sample

Product Details

Description

SIRT4 Antibody (YA6962) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Recombinant protein within human SIRT4 aa 1-314.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SIRT4 Antibody (YA6962)
    Western blot analysis of extracts from RAW264.7 (lane 1(20μg)) and Rat kidney (lane 2(20μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) and Human kidney (lane 2(40μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SIRT4 is a mitochondrial member of the sirtuin family that functions as an NAD+-dependent metabolic regulator and is distinguished by its prominent ADP-ribosyltransferase activity toward glutamate dehydrogenase (GDH) [1][2]. Mechanistically, SIRT4 represses mitochondrial glutamine and glutamate metabolism through ADP-ribosylation-mediated inhibition of GDH, thereby reducing amino acid-stimulated insulin secretion and modulating cellular energy homeostasis[1][2][3]. Beyond GDH regulation, SIRT4 participates in broader metabolic control, including the regulation of fatty acid oxidation, branched-chain amino acid catabolism, and mitochondrial metabolic adaptation during nutrient stress[4][5][6]. These functions position SIRT4 as an important regulator of mitochondrial metabolism and endocrine signaling in pancreatic β cells and other metabolically active tissues[1][4]. In disease contexts, reduced SIRT4 activity or expression has been associated with enhanced glutamine utilization and tumor progression, whereas restoration of SIRT4 suppresses proliferation and promotes tumor-suppressive metabolic programs in multiple experimental cancer models[7][8][9]. Compared with related mitochondrial isoforms such as SIRT3 and SIRT5, which primarily function through deacylation-dependent activation or remodeling of metabolic enzymes, SIRT4 is notable for suppressing metabolic flux through ADP-ribosylation-mediated inhibition of key mitochondrial targets[1][10]. For experimental applications, selective SIRT4 inhibitors have recently been reported, providing emerging tools for mechanistic studies of mitochondrial metabolism, insulin secretion, and cancer-associated metabolic reprogramming[11].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_Specificity: Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus
    Induction: Induced by glutamine (at protein level)

  • Isoforms & Post-Translational Modification

    Q9Y6E7: 314 amino acids, molecular weight 35188 Da.

  • Subunit

    Interacts with GLUD1, IDE and SLC25A5 (PubMed:16959573, PubMed:17715127)

  • SwissProt ID

    Q9Y6E7

  • Synonyms

    MGC130046 antibody; MGC130047 antibody; MGC57437 antibody; NAD dependent deacetylase sirtuin 4 antibody; NAD dependent protein deacetylase sirtuin 4 antibody; NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody; NAD-dependent protein deacetylase sirtuin-4 antibody; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial antibody; Regulatory protein SIR2 homolog 4 antibody; Silent mating type information regulation 2 homolog antibody

References

[1]. Haigis MC, et al. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. Cell. 2006 Sep 8;126(5):941-54. [Content Brief]

[2]. Caride AJ, et al. The plasma membrane Ca2+ pump isoform 4a differs from isoform 4b in the mechanism of calmodulin binding and activation kinetics: implications for Ca2+ signaling. J Biol Chem. 2007 Aug 31;282(35):25640-8. [Content Brief]

[3]. Zaganjor E, et al. SIRT4 Is a Regulator of Insulin Secretion. Cell Chem Biol. 2017;24(6):656-658.

[4]. Min Z, et al. The Roles of Mitochondrial SIRT4 in Cellular Metabolism. Front Endocrinol (Lausanne). 2019;9:783.

[5]. Zaganjor E, et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis. Cell Rep. 2021 Jul 13;36(2):109345. [Content Brief]

[6]. Wood JG, et al. Sirt4 is a mitochondrial regulator of metabolism and lifespan in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1564-1569. [Content Brief]

[7]. Li J, et al. Sirtuin 4 activates autophagy and inhibits tumorigenesis by upregulating the p53 signaling pathway. Cell Death Differ. 2023;30:313-326.

[8]. Cai G, et al. SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism. Sci Rep. 2022 Jul 16;12(1):12208. [Content Brief]

[9]. Wang C, et al. Mammalian SIRT4 is a tumor suppressor of clear cell renal cell carcinoma by inhibiting cancer proliferation, migration and invasion. Cancer Biomark. 2020;29(4):453-462. [Content Brief]

[10]. Kim E A, et al. Inhibition of glutamate dehydrogenase and insulin secretion by KHG26377 does not involve ADP-ribosylation by SIRT4 or deacetylation by SIRT3[J]. Bmb Reports, 2012, 45(8): 458-463.

[11]. Pannek M, et al. Specific Inhibitors of Mitochondrial Deacylase Sirtuin 4 Endowed with Cellular Activity. J Med Chem. 2024 Feb 8;67(3):1843-1860. [Content Brief]

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SIRT4 Antibody (YA6962) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 1(20μg)) and Rat kidney (lane 2(20μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) and Human kidney (lane 2(40μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SIRT4 Antibody (YA6962) Product Image
    1/16
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • SIRT4 Antibody (YA6962) Product Image
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 1(20μg)) and Rat kidney (lane 2(20μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) and Human kidney (lane 2(40μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • Experimental Validation Results for SIRT4 Antibody (YA6962)
  • SIRT4 Antibody (YA6962) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7 (lane 1(20μg)) and Rat kidney (lane 2(20μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SIRT4 Antibody (YA6962) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) and Human kidney (lane 2(40μg)) using SIRT4 Antibody (HY-P87279) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SIRT4 Antibody (YA6962) Product Image WB
  • SIRT4 Antibody (YA6962) Product Image WB