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RBM10 Antibody (YA8948)

(Synonyms: DXS8237E, GPATC9, GPATCH9, KIAA0122, RBM10, RNA-binding protein 10, G patch domain-containing protein 9, RNA-binding motif protein 10, RNA-binding protein S1-1, S1-1)

RBM10 Antibody (YA8948) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBM10.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RBM10 Antibody (YA8948)
  • RBM10 Antibody (YA8948) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:50-3000

Product Details

Description

RBM10 Antibody (YA8948) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBM10.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 103 kDa
Species Reactivity Database

OMIM: 300080

Immunogen

A synthesized peptide derived from human RBM10(aa437-570).

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RBM10 Antibody (YA8948)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3,), 293T (lane 4), MCF-7 (lane 5, Negative), Jurkat (lane 6, Negative), and K562 (lane 7) using RBM10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:200) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    RBM10 binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A) (By similarity). May bind to specific miRNA hairpins[1][2][3].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    RBM10 has 6 isoforms, P98175-1: amino acid length is 930, molecular weight is 103533 Da (predicted); P98175-2: amino acid length is 929, molecular weight is 103433 Da (predicted); P98175-3: amino acid length is 853, molecular weight is 94469 Da (predicted); P98175-4: amino acid length is 852, molecular weight is 94370 Da (predicted); P98175-5: amino acid length is 995, molecular weight is 110366 Da (predicted); P0DW28-1: amino acid length is 130, molecular weight is 14216 Da (predicted).

  • Subunit

    Associates with the spliceosome.

  • SwissProt ID

    P98175

  • Gene ID
    8241 [NCBI]
  • Synonyms

    DXS8237E, GPATC9, GPATCH9, KIAA0122, RBM10, RNA-binding protein 10, G patch domain-containing protein 9, RNA-binding motif protein 10, RNA-binding protein S1-1, S1-1

References

[1]. Nguyen CD, et al. Characterization of a family of RanBP2-type zinc fingers that can recognize single-stranded RNA. J Mol Biol. 2011 Mar 25;407(2):273-83. [Content Brief]

[2]. Inoue A, et al. S1-1 nuclear domains: characterization and dynamics as a function of transcriptional activity. Biol Cell. 2008 Sep;100(9):523-35. [Content Brief]

[3]. Treiber T, et al. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Mol Cell. 2017 Apr 20;66(2):270-284.e13. [Content Brief]

RBM10 Antibody (YA8948) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3,), 293T (lane 4), MCF-7 (lane 5, Negative), Jurkat (lane 6, Negative), and K562 (lane 7) using RBM10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:200) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RBM10 Antibody (YA8948) Product Image
    1/16
  • Experimental Validation Results for RBM10 Antibody (YA8948)
  • RBM10 Antibody (YA8948) Product Image
  • Experimental Validation Results for RBM10 Antibody (YA8948)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3,), 293T (lane 4), MCF-7 (lane 5, Negative), Jurkat (lane 6, Negative), and K562 (lane 7) using RBM10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:200) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for RBM10 Antibody (YA8948)
  • RBM10 Antibody (YA8948) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3,), 293T (lane 4), MCF-7 (lane 5, Negative), Jurkat (lane 6, Negative), and K562 (lane 7) using RBM10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:200) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RBM10 Antibody (YA8948) Product Image WB