PMF 1 Antibody (YA3165)(PBS only)

Cat. No.: HY-P83420A: Data Sheet User Guide for Antibodies
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PMF 1 Antibody (YA3165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMF 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PMF 1 Antibody (YA3165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMF 1.

Host

Rabbit

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q6P1K2

Gene ID

100527963 [NCBI]

Immunogen

Recombinant protein of human PMF-1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT

Subcellular Localization：Nucleus; Chromosome, centromere, kinetochore

Expression：
Tissue_specificity:Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver

Induction:By polyamine analogs in analog-sensitive H157 cells

Isoforms & Post-Translational Modification：Q6P1K2 has 6 isomers: Q6P1K2-1: 23339 Da (predicted); Q6P1K2-2: 22946 Da (predicted); Q6P1K2-3: 18977 Da (predicted); Q6P1K2-4: 19238 Da (predicted); Q6P1K2-5: 24032 Da (predicted); Q6P1K2-6: 20230 Da (predicted).

Subunit：Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Interacts with COPS7A. Interacts via its coiled-coil domain with the leucine-zipper domain of NFE2L2. The interaction with NFE2L2 is required for the transcriptional regulation of SSAT

Research Field

Synonyms

Polyamine-modulated factor 1

Documentation

User Guide for Antibodies (1077 KB)