Acetylcholinesterase Antibody (YA9876)
(Synonyms: ACEE; ACES_HUMAN; Acetylcholinesterase; AChE; Apoptosis related acetylcholinesterase; ARACHE; N ACHE; N-ACHE; YT; YT blood group;)Based on 1 Customer Validation
Acetylcholinesterase Antibody (YA9876) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Acetylcholinesterase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P
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Reactivity :
Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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|---|---|---|---|
| Dilution Ratio | 1:5000-20000 | 1:100 | 1:500-1000 |
Product Details
Acetylcholinesterase Antibody (YA9876) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Acetylcholinesterase.
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Host Rabbit
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Species ReactivityMouse, Rat
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Observed Molecular WeightObserved band size: 68 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
Synthetic peptide of human Acetylcholinesterase.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Rat brain tissue (lane 2, 40ug), Mouse liver tissue (lane3, 40μg) using Acetylcholinesterase Antibody (HY-P810614). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
Acetylcholinesterase is a Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft
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Subcellular Localization
Synapse; Secreted; Cell membrane
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Expression
Tissue_Specificity: Predominates in most expressing tissues except erythrocytes where a glycophospholipid-attached form of ACHE predominates. -
Isoforms & Post-Translational Modification
Acetylcholinesterase has 2 isoforms, P21836-1: amino acid length is 614, molecular weight is 68169 Da (predicted); P21836-2: amino acid length is ?, molecular weight is ? Da Da (predicted).
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Subunit
Isoform H generates GPI-anchored dimers; disulfide linked.
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SwissProt ID
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Synonyms
ACEE; ACES_HUMAN; Acetylcholinesterase; AChE; Apoptosis related acetylcholinesterase; ARACHE; N ACHE; N-ACHE; YT; YT blood group;
Documentation