MYO6 Antibody
(Synonyms: KIAA0389, MYO6, Unconventional myosin-VI, Unconventional myosin-6)MYO6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MYO6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
MYO6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MYO6.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 149 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 149 kDa
Synthetic peptide corresponding to the N-term region of human MYO6.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MYO6 is a Myosins are actin-based motor molecules with ATPase activity (By similarity). Unconventional myosins serve in intracellular movements (By similarity). Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding (By similarity). Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration (By similarity). Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. Together with TOM1, mediates delivery of endocytic cargo to autophagosomes thereby promoting autophagosome maturation and driving fusion with lysosomes. Links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN. May act as a regulator of F-actin dynamics (By similarity). As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets (By similarity). May play a role in the extension and network organization of neurites (By similarity). Required for structural integrity of inner ear hair cells (By similarity). Required for the correct localization of CLIC5 and RDX at the stereocilium base (By similarity). Modulates RNA polymerase II-dependent transcription[1][2][3][4][5][6][7].
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Subcellular Localization
Golgi apparatus, trans-Golgi network membrane; Golgi apparatus; Nucleus; Cytoplasm, perinuclear region; Membrane, clathrin-coated pit; Cytoplasmic vesicle, clathrin-coated vesicle; Cell projection, filopodium; Cell projection, ruffle membrane; Cell projection, microvillus; Cytoplasm, cytosol; Cytoplasmic vesicle, autophagosome; Endosome
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Expression
Tissue_Specificity: Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells. -
Isoforms & Post-Translational Modification
MYO6 has 6 isoforms, Q9UM54-3: amino acid length is 1294, molecular weight is 149691 Da (predicted); Q9UM54-1: amino acid length is 1285, molecular weight is 148714 Da (predicted); Q9UM54-2: amino acid length is 1262, molecular weight is 146048 Da (predicted); Q9UM54-4: amino acid length is 1285, molecular weight is 148685 Da (predicted); Q9UM54-5: amino acid length is 1253, molecular weight is 145042 Da (predicted); Q9UM54-6: amino acid length is 1285, molecular weight is 148659 Da (predicted).
Phosphorylation in the motor domain, induced by EGF, results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK). -
Subunit
Homodimer; dimerization seems to implicate the unfolding of the three-helix bundle region creating an additional calmodulin binding site, and cargo binding (By similarity).
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SwissProt ID
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Synonyms
KIAA0389, MYO6, Unconventional myosin-VI, Unconventional myosin-6
Documentation
References
[1]. Wells AL, et al. Myosin VI is an actin-based motor that moves backwards. Nature. 1999 Sep 30;401(6752):505-8. [Content Brief]
[2]. Jung EJ, et al. Myosin VI is a mediator of the p53-dependent cell survival pathway. Mol Cell Biol. 2006 Mar;26(6):2175-86. [Content Brief]
[3]. Buss F, et al. Myosin VI isoform localized to clathrin-coated vesicles with a role in clathrin-mediated endocytosis. EMBO J. 2001 Jul 16;20(14):3676-84. [Content Brief]
[4]. Tumbarello DA, et al. Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nat Cell Biol. 2012 Oct;14(10):1024-35. [Content Brief]
[5]. Hu S, et al. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. Nat Commun. 2019 Aug 1;10(1):3459. [Content Brief]
[6]. O'Loughlin T, et al. The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics. EMBO Rep. 2018 Apr;19(4):. [Content Brief]
[7]. Vreugde S, et al. Nuclear myosin VI enhances RNA polymerase II-dependent transcription. Mol Cell. 2006 Sep 1;23(5):749-55. [Content Brief]
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