IDOL Antibody
(Synonyms: BZF1, IDOL, BM-023, PP5242, MYLIP, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein, RING-type E3 ubiquitin transferase MYLIP, Idol, MIR)IDOL Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IDOL.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
IDOL Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IDOL.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 49 kDa
Synthetic peptide corresponding to the center region of human IDOL.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IDOL is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR
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Subcellular Localization
Cytoplasm; Cell membrane
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Expression
Tissue_Specificity: Ubiquitously expressed.
Induction: Expression is directly activated by NR1H2 and NR1H3. Expression is not dependent of the sterol-response element-binding proteins (SREBPs). Expression is indirectly induced by LDL. -
Isoforms & Post-Translational Modification
IDOL has 2 isoforms, Q8WY64-1: amino acid length is 445, molecular weight is 49910 Da (predicted); Q8WY64-2: amino acid length is 134, molecular weight is 14989 Da (predicted).可发生自泛素化IDOL 存在 2 个异构体，Q8WY64-1：氨基酸个数为 445 个，分子量为 49910 Da (预测)；Q8WY64-2：氨基酸个数为 134 个，分子量为 14989 Da (预测)。Autoubiquitinated
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
BZF1, IDOL, BM-023, PP5242, MYLIP, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein, RING-type E3 ubiquitin transferase MYLIP, Idol, MIR
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