Tryptophan tRNA Ligase Antibody (YA1608)
(Synonyms: Gamma2; hWRS; IFI53; IFP53; TrpRS; WARS; WRS)Tryptophan tRNA Ligase Antibody (YA1608) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tryptophan tRNA Ligase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 | 1:20 |
Product Details
Tryptophan tRNA Ligase Antibody (YA1608) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tryptophan tRNA Ligase.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 53 kDa
A synthetic peptide of human Tryptophanyl tRNA synthetase
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Tryptophan tRNA Ligase catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp); Has no angiostatic activity; Possesses an angiostatic activity but has no aminoacylation activity. Inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression; Has an angiostatic activity[1][2][3].
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Subcellular Localization
Cytoplasm
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Expression
Induction:By IFNG/IFN-gamma -
Isoforms & Post-Translational Modification
P23381 has 2 isomers: P23381-1: 53165 Da (predicted); P23381-2: 48852 Da (predicted).
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS -
Subunit
Homodimer (PubMed:28369220). Interacts with an oxidized form of GAPDH. GAPDH stimulates the aminoacylation activity of isoform 2 (PubMed:15628863)
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SwissProt ID
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Synonyms
Gamma2; hWRS; IFI53; IFP53; TrpRS; WARS; WRS
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Otani A, et al. A fragment of human TrpRS as a potent antagonist of ocular angiogenesis. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):178-83. [Content Brief]
[2]. Wakasugi K, et al. A human aminoacyl-tRNA synthetase as a regulator of angiogenesis. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):173-7. [Content Brief]
[3]. Tzima E, et al. Biologically active fragment of a human tRNA synthetase inhibits fluid shear stress-activated responses of endothelial cells. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14903-7. [Content Brief]
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