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CD322 Antibody (YA4111)

(Synonyms: JAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43)
Customer Review

Based on 1 Customer Validation

CD322 Antibody (YA4111) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD322.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • CD322 Antibody (YA4111) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100 1:200-1:400 1:10000

Product Details

Description

CD322 Antibody (YA4111) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD322.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 33-43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD322 Antibody (YA4111)
    Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), HL-60 (lane4(20μg) and Ramos (lane5(20μg) using CD322 Antibody (HY-P84414). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD322 Antibody (YA4111)
    Flow cytometric analysis of 1X106 HeLa cells labeling CD322 Antibody (HY-P84414, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD322 regulates a variety of cellular processes by mediating interactions between heterologous cells through its homologous receptor JAM3. CD322 plays a role in the homing and mobilization of hematopoietic stem cells and progenitors in the bone marrow. On the surface of bone marrow stromal cells, CD322 facilitates the retention of hematopoietic stem cells and progenitors expressing JAM3. CD322 plays a central role in leukocyte exudation, promoting not only transendothelial migration of leukocytes but also adhesion and rolling of leukocytes along endothelial cells. Leukocyte adhesion and rolling depend on the binding of integrin α4/β1 to CD322[1].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell junction; Cell junction, tight junction

  • Expression


    Tissue_specificity:Highly expressed in heart, placenta, lung, foreskin and lymph node (PubMed:10779521, PubMed:10945976) . Prominently expressed on high endothelial venules and also present on the endothelia of other vessels (at protein level) (PubMed:10779521, PubMed:10945976) . Also expressed in the brain in the caudate nuclei (PubMed:31851307)

  • Isoforms & Post-Translational Modification

    CD322 has 3 isomers: P57087-1: 33207 Da (predicted); P57087-2: 29037 Da (predicted); P57087-3: 34554 Da (predicted).

  • SwissProt ID

    P57087

  • Gene ID
    58494 [NCBI]
  • Synonyms

    JAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43

References

[1]. Arrate MP, et al. Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor. J Biol Chem. 2001 Dec 7;276(49):45826-32. [Content Brief]

CD322 Antibody (YA4111) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), HL-60 (lane4(20μg) and Ramos (lane5(20μg) using CD322 Antibody (HY-P84414). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD322 Antibody (HY-P84414, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD322 Antibody (YA4111) Product Image
    1/16
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • CD322 Antibody (YA4111) Product Image
  • Experimental Validation Results for CD322 Antibody (YA4111)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), HL-60 (lane4(20μg) and Ramos (lane5(20μg) using CD322 Antibody (HY-P84414). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD322 Antibody (YA4111)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD322 Antibody (HY-P84414, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • Experimental Validation Results for CD322 Antibody (YA4111)
  • CD322 Antibody (YA4111) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), HL-60 (lane4(20μg) and Ramos (lane5(20μg) using CD322 Antibody (HY-P84414). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • CD322 Antibody (YA4111) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling CD322 Antibody (HY-P84414, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD322 Antibody (YA4111) Product Image WB
  • CD322 Antibody (YA4111) Product Image FC