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Oncostatin M Antibody (YA2813)

(Synonyms: Oncostatin-M (OSM))
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Based on 1 Customer Validation

Oncostatin M Antibody (YA2813) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Oncostatin M.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Oncostatin M Antibody (YA2813) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Oncostatin M Antibody (YA2813) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Oncostatin M.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Oncostatin M aa36-120.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Oncostatin M/OSM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), Jurkat (lane 5(20μg) using Oncostatin M Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Oncostatin M is a Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration

  • Subcellular Localization

    Secreted

  • SwissProt ID

    P13725

  • Gene ID
    5008 [NCBI]
  • Synonyms

    Oncostatin-M (OSM)

  • Research Field

    Immunology

Oncostatin M Antibody (YA2813) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Oncostatin M/OSM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), Jurkat (lane 5(20μg) using Oncostatin M Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Oncostatin M Antibody (YA2813) Product Image
    1/16
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Oncostatin M Antibody (YA2813) Product Image
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Oncostatin M/OSM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), Jurkat (lane 5(20μg) using Oncostatin M Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Experimental Validation Results for Oncostatin M Antibody (YA2813)
  • Oncostatin M Antibody (YA2813) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using Oncostatin M/OSM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Oncostatin M Antibody (YA2813) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), Jurkat (lane 5(20μg) using Oncostatin M Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Oncostatin M Antibody (YA2813) Product Image WB
  • Oncostatin M Antibody (YA2813) Product Image WB