ACE2 Antibody (YA6160)

Cat. No.: HY-P86468: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

ACE2 Antibody (YA6160) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACE2.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

ACE2 Antibody (YA6160) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACE2.

Host

Rabbit

Clonality

Monoclonal

Peso molecular

Predicted band size: 130 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BYF1

Gene ID

59272 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:4000
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Pureza	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582); (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63; Non-functional as a carboxypeptidase; (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2

Subcellular Localization：Secreted; Cell membrane; Single-pass type I membrane protein; Cytoplasm; Cell projection, cilium; Apical cell membrane; Apical cell membrane

Expression：
Tissue_specificity:It is expressed (protein level) in endothelial cells and arterial smooth muscle cells of large and small arteries (PubMed:15141377) . It is also expressed (protein level) in intestinal cells, Leydig cells, and Sertoli cells (PubMed:15141377) . Furthermore, it is expressed (protein level) in the proximal tubules of the kidney and small intestine (PubMed:18424768) . This gene is also expressed (protein level) in the heart, kidney, testis, and gastrointestinal tract (PubMed:10924499, PubMed:10969042, PubMed:12459472, PubMed:15231706, PubMed:15671045, PubMed:32170560, PubMed:32715618) . In the lungs, this gene is expressed at low levels in some type II alveolar cells, and its expression appears to be individual-specific (protein level) (PubMed:15141377, PubMed:32170560, PubMed:32425701, PubMed:32715618, PubMed:33432184) . It is also expressed in nasal epithelial cells (protein level) (PubMed:32333915, PubMed:33432184) . It is co-expressed with TMPRSS2 in some type II alveolar cells, ileal absorptive intestinal cells, intestinal epithelial cells, cornea, gallbladder, and nasal goblet cells (PubMed:32327758, PubMed:32358202, PubMed:32413319) . Co-expressed with TMPRSS4 in mature intestinal cells (PubMed:32404436) ; expressed in nasal and bronchial epithelial cells (protein level) .

Induction:Up-regulated in failing heart (PubMed:14504186, PubMed:15151696, PubMed:15671045) . Expression is induced by IFNA and IFNG (PubMed:32413319, PubMed:32425701) .

Isoforms & Post-Translational Modification：Q9BYF1 has 2 isomers: Q9BYF1-1: 92463 Da (predicted); Q9BYF1-3: 52737 Da (predicted).
N-glycosylation on Asn-90 may limit SARS infectivity;Proteolytic cleavage by ADAM17 generates a secreted form (PubMed:15983030, PubMed:33713620). Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1;Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains;Ubiquitinated. Ubiquitinated on Lys-788 via 'Lys-48'-linked ubiquitin (PubMed:36876523). 'Lys-48'-linked deubiquitinated by USP50 on the Lys-788; leading to its stabilization (PubMed:36876523)

Subunit：Homodimer (PubMed:32132184). Interacts with the catalytically active form of TMPRSS2 (PubMed:21068237). Interacts with SLC6A19; this interaction is essential for expression and function of SLC6A19 in intestine (By similarity). Interacts with ITGA5:ITGB1 (PubMed:15276642, PubMed:33102950). Probably interacts (via endocytic sorting signal motif) with AP2M1; the interaction is inhibited by phosphorylation of Tyr-781 (PubMed:33436498). Interacts (via PDZ-binding motif) with NHERF1 (via PDZ domains); the interaction may enhance ACE2 membrane residence (PubMed:34189428)

Synonyms

Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15; [Cleaved into: Processed angiotensin-converting enzyme 2]

Documentación

User Guide for Antibodies (1077 KB)