LDLR Antibody (YA3452)

Cat. No.: HY-P83717: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

LDLR Antibody (YA3452) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LDLR.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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LDLR Antibody (YA3452) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

LDLR Antibody (YA3452) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LDLR.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 92kDa;

Observed band size: 140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P01130

Gene ID

3949 [NCBI]

Immunogen

A synthesized peptide derived from human LDL Receptor aa823-860.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	affinity purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HuH-7 (lane 1, 15 μg), and HepG2 (lane 2, 15 μg) using LDL Receptor Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using LDLR Antibody (HY-P83717) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization (PubMed:30443021); (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins; (Microbial infection) Acts as a receptor for Vesicular stomatitis virus; (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells; (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV); (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane, clathrin-coated pit; Golgi apparatus; Early endosome; Late endosome; Lysosome

Isoforms & Post-Translational Modification：P01130 has 6 isomers: P01130-1: 95376 Da (predicted); P01130-2: 75854 Da (predicted); P01130-3: 76856 Da (predicted); P01130-4: 90940 Da (predicted); P01130-5: 95092 Da (predicted); P01130-6: 82255 Da (predicted).
N- and O-glycosylated;Ubiquitinated by MYLIP leading to degradation

Subunit：Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts (via NPXY motif) with LDLRAP1 (via PID domain) (PubMed:12221107, PubMed:22509010). Interacts with ARRB1 (PubMed:12944399). Interacts with SNX17 (PubMed:14739284). Interacts with the full-length immature form of PCSK9 (via C-terminus) (PubMed:17461796, PubMed:21149300). Interacts with PGRMC1 and TMEM97; the interaction increases LDL internalization (PubMed:30443021)

RRID

AB_3676589

Database

Entrez Gene: 3949 Human ; 16835 Mouse ;

SwissProt: P01130 Human ; P35951 Mouse ;

OMIM: 143890 Human

Research Field

Cardiovascular

Synonyms

LDLR_HUMAN; Low-density lipoprotein receptor; LDL receptor; FH; FHC; FHCL1; LDLCQ2; low density lipoprotein receptor;

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

LDLR Antibody (YA3452) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LDLR Antibody (YA3452)

LDLR Antibody (YA3452) Featured Recommendations:

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Background

Documentation

LDLR Antibody (YA3452) Related Classifications

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