Aquaporin 5 Antibody (YA6478)

Cat. No.: HY-P86785: Data Sheet COA
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Aquaporin 5 Antibody (YA6478) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aquaporin 5.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Aquaporin 5 Antibody (YA6478) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aquaporin 5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P55064

Gene ID

362 [NCBI]

Immunogen

Synthetic peptide within human Aquaporin 5 aa 225-265/265.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:500
mIHC mIHC: Multiplex Immunohistochemical	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Human lung (lane 1(20μg)) using Aquaporin 5 Antibody (HY-P86785) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed:18768791, PubMed:8621489). Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity)

Subcellular Localization：Apical cell membrane,Cell membrane,Cytoplasmic vesicle membrane

Expression：
Tissue_Specificity: Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level)

Isoforms & Post-Translational Modification：P55064: 265 amino acids, molecular weight 28292 Da.

Subunit：Homotetramer; each monomer provides an independent water pore (PubMed:18768791, PubMed:26569106)

RRID

AB_3719210

Synonyms

AQP 5 antibody; AQP-5 antibody; AQP5 antibody; AQP5_HUMAN antibody; Aquaporin 5 antibody; Aquaporin-5 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)