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Vinculin Antibody (YA5517)

(Synonyms: VCL; Vinculin; Metavinculin)
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Based on 1 Customer Validation

Vinculin Antibody (YA5517) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Vinculin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Vinculin Antibody (YA5517)
  • Vinculin Antibody (YA5517) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

Vinculin Antibody (YA5517) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Vinculin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 124 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 124 kDa
Immunogen

Synthesized peptide derived from human Vinculin. AA range: 100-200

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3719052

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vinculin Antibody (YA5517)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C6 (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Vinculin Antibody (HY-P85825) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Vinculin is an Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, adherens junction; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side; Cell projection, podosome

  • Expression


    Tissue_specificity:Metavinculin is a muscle-specific protein.

  • Isoforms & Post-Translational Modification

    P18206 has 3 isomers: P18206-1: 123799 Da (predicted); P18206-2: 116722 Da (predicted); P18206-3: 24904 Da (predicted).
    Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity);Acetylated; mainly by myristic acid but also by a small amount of palmitic acid

  • Subunit

    Exhibits self-association properties. Part of a complex composed of THSD1, PTK2/FAK1, TLN1 and VCL (PubMed:29069646).

  • SwissProt ID

    P18206

  • Gene ID
    7414 [NCBI]
  • Synonyms

    VCL; Vinculin; Metavinculin

Vinculin Antibody (YA5517) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C6 (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Vinculin Antibody (HY-P85825) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Vinculin Antibody (YA5517) Product Image
    1/16
  • Experimental Validation Results for Vinculin Antibody (YA5517)
  • Vinculin Antibody (YA5517) Product Image
  • Experimental Validation Results for Vinculin Antibody (YA5517)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C6 (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Vinculin Antibody (HY-P85825) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vinculin Antibody (YA5517)
  • Vinculin Antibody (YA5517) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C6 (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Vinculin Antibody (HY-P85825) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Vinculin Antibody (YA5517) Product Image WB