Drebrin Antibody (YA3318)

製品番号: HY-P83573: データシート SDS COA User Guide for Antibodies
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Drebrin Antibody (YA3318) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Drebrin.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Drebrin Antibody (YA3318) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Drebrin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 71 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q16643

Gene ID

1627 [NCBI]

Immunogen

A synthesized peptide derived from human Drebrin aa120-181/649.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using Drebrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (PubMed:20215400). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (PubMed:20215400). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity)

Subcellular Localization：Cytoplasm; Cell projection, dendrite; Cytoplasm, cell cortex; Cell junction; Cell projection, growth cone

Expression：
Tissue_specificity:Expressed in the brain, with expression in the molecular layer of the dentate gyrus, stratum pyramidale, and stratum radiatum of the hippocampus (at protein level) (PubMed:8838578) . Also expressed in the terminal varicosities distributed along dendritic trees of pyramidal cells in CA4 and CA3 of the hippocampus (at protein level) (PubMed:8838578) . Expressed in pyramidal cells in CA2, CA1 and the subiculum of the hippocampus (at protein level) (PubMed:8838578) . Expressed in peripheral blood lymphocytes, including T-cells (at protein level) (PubMed:20215400) . Expressed in the brain (PubMed:8216329, Ref.2) . Expressed in the heart, placenta, lung, skeletal muscle, kidney, pancreas, skin fibroblasts, gingival fibroblasts and bone-derived cells (Ref.2)

Isoforms & Post-Translational Modification：Q16643 has 3 isomers: Q16643-1: 71429 Da (predicted); Q16643-2: 71599 Da (predicted); Q16643-3: 76300 Da (predicted).

Subunit：Interacts with RUFY3 (By similarity). Interacts with CXCR4; this interaction is enhanced by antigenic stimulation (PubMed:20215400).

RRID

AB_3105681

Database

Entrez Gene: 1627 Human ; 56320 Mouse ; 81653 Rat

SwissProt: Q16643 Human ; Q9QXS6 Mouse ; Q07266 Rat

OMIM: 104300 Human

Research Field

Neuroscience

Synonyms

D0S117E; DBN1; Drebrin 1; Drebrin; Drebrin E2; Drebrin1; DrebrinE

ドキュメンテーション

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データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)