DCTN4 Antibody (YA4569)

Cat. No.: HY-P84872: Data Sheet User Guide for Antibodies
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DCTN4 Antibody (YA4569) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DCTN4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DCTN4 Antibody (YA4569) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DCTN4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UJW0

Gene ID

51164 [NCBI]

Immunogen

Purified recombinant fragment of human DCTN4 (AA: 57-298) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, cell cortex; Cytoplasm, myofibril, sarcomere

Isoforms & Post-Translational Modification：Q9UJW0 has 3 isomers: Q9UJW0-1: 52337 Da (predicted); Q9UJW0-2: 45757 Da (predicted); Q9UJW0-3: 53152 Da (predicted).

Subunit：Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin (By similarity). Interacts with ATP7B, but not ATP7A, in a copper-dependent manner (PubMed:16554302). Interacts with ANK2; this interaction is required for localization at costameres (By similarity). Interacts with N4BP2L1 (By similarity)

Synonyms

P62; DYN4

Documentation

User Guide for Antibodies (1077 KB)