2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. NELFB Antibody (YA7271)

NELFB Antibody (YA7271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFB.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

NELFB Antibody (YA7271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFB.
Host

Rabbit
Clonality

Recombinant,Monoclonal
分子量
Predicted band size: 66 kDa;
Observed band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Predicted Reactivity

Human

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
SwissProt ID
Immunogen

A synthesized peptide derived from human NELF B
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-200
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-200
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:50-200
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
Sensitivity Endogenous 純度 affinity purified by Protein A
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401). May be able to induce chromatin unfolding (PubMed:11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity)|(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed:23884411). In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed:23884411)
Subcellular Localization：Nucleus
Expression：
Tissue_Specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas
Isoforms & Post-Translational Modification：Q8WX92 has two isomers: Q8WX92-1: 65697 Da (predicted); Q8WX92-2: 70057 Da (predicted).
Subunit：The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex (PubMed:12612062)
ドキュメンテーション
User Guide for Antibodies (1077 KB)

NELFB Antibody (YA7271) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
NELFB Antibody (YA7271)
製品番号:
HY-P87587
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