PIP5K1C Antibody (YA1644)
(Synonyms: AI115456; AI835305; Pip5kIgamma)PIP5K1C Antibody (YA1644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIP5K1C.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
PIP5K1C Antibody (YA1644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIP5K1C.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 90,87 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 72 kDa
Entrez Gene: 23396 Human ; 18717 Mouse ; 314641 Rat
SwissProt: O60331 Human ; O70161 Mouse ; Q5I6B8 Rat
OMIM: 611369 Human
Recombinant protein of mouse PIP5KI gamma
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIP5K1C encodes phosphatidylinositol-4-phosphate 5-kinase type 1γ (PIPKIγ), a lipid kinase that catalyzes the conversion of phosphatidylinositol 4-phosphate into phosphatidylinositol 4, 5-bisphosphate (PIP2), a key second messenger regulating signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility[1][2]. Mechanistically, PIP5K1C organizes localized PIP2 pools at focal adhesions and other membrane-associated signaling domains, thereby coordinating integrin-mediated adhesion and cytoskeletal remodeling[3][1]. Through direct association with talin, PIPKIγ is recruited to focal adhesions, where it promotes integrin-talin coupling and regulates focal adhesion assembly and turnover[3][1][4]. In addition to its adhesion-related functions, PIP5K1C contributes to clathrin-dependent endocytosis, synaptic vesicle trafficking, and directional cell migration, highlighting its central role in membrane dynamics and cellular signaling[1][2]. Disease studies further demonstrate that altered PIP5K1C activity influences tumor-associated signaling pathways, including AKT-STAT3 signaling and cancer cell invasion, while genetic deficiency causes developmental abnormalities and tissue-specific defects in experimental models[4][5]. Compared with the related isoforms PIP5K1A and PIP5K1B, PIP5K1C exhibits distinctive localization to focal adhesions and specialized interactions with talin, supporting nonredundant functions in adhesion-dependent signaling and migration[3][1][4]. Although PIP5K1C remains an attractive therapeutic target, selective pharmacological modulators are currently less established than those available for other lipid signaling enzymes, making genetic and mechanistic approaches the primary tools for experimental investigation[4][5].
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Subcellular Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side; Endomembrane system; Cytoplasm; Cell junction, focal adhesion; Cell junction, adherens junction; Cell projection, ruffle membrane; Cell projection, phagocytic cup; Cell projection, uropodium
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Expression
Tissue_specificity:High expression in brain. Also detected in lung, thymus, heart, testicle, kidney and embryo. Highly expressed in forebrain, in particular in cerebellum, hippocampus and cerebral cortex -
Isoforms & Post-Translational Modification
O70161 has 3 isomers: O70161-1: 72408 Da (predicted); O70161-2: 69199 Da (predicted); O70161-3: 69502 Da (predicted).
Phosphorylation on Ser-645 negatively regulates binding to TLN2 and is strongly stimulated in mitosis. Phosphorylation on Tyr-644 is necessary for targeting to focal adhesions. Phosphorylation on Ser-645 and Tyr-644 are mutually exclusive. Phosphorylated by SYK and CSK. Tyrosine phosphorylation is enhanced by PTK2 signaling. Phosphorylated at Tyr-634 upon EGF stimulation. Some studies suggest that phosphorylation on Tyr-644 enhances binding to tailins (TLN1 and TLN2); others that phosphorylation at Tyr-644 does not directly enhance binding to tailins (TLN1 and TLN2) but may act indirectly by inhibiting phosphorylation at Ser-645;Acetylation at Lys-265 and Lys-268 seems to decrease lipid kinase activity. Deacetylation of these sites by SIRT1 positively regulates the exocytosis of TSH-containing granules from pituitary cells (By similarity) -
Subunit
Isoform 1 interacts with TLN1 (PubMed:12422220, PubMed:14691141, PubMed:16707488).
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SwissProt ID
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Synonyms
AI115456; AI835305; Pip5kIgamma
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Research Field
Signal Transduction
Documentation
References
[1]. Ling K, et al. Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions. Nature. 2002 Nov 7;420(6911):89-93. [Content Brief]
[2]. Uniprotkb.
[3]. Di Paolo G, et al. Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin. Nature. 2002 Nov;420(6911):85-89. [Content Brief]
[4]. PIP5K1C gene information from NCBI.
[5]. Yan Q, et al. Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling. J Biol Chem. 2022 Mar;298(3):101639. [Content Brief]
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