P2RY1 Antibody (YA4040)
(Synonyms: P2Y1)P2RY1 Antibody (YA4040) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RY1.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
P2RY1 Antibody (YA4040) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RY1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
Purified recombinant fragment of human P2RY1 (AA: extra mix) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
P2RY1 is a Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition; Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response. Also blocks IL11 cluster signaling through IL11R[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:Found in all the tissues and cell lines examined (PubMed:2261637) . Expression not restricted to IL6 responsive cells (PubMed:2261637) ; Expressed in blood serum (at protein level) (PubMed:24629561)
Induction:LIF and OSM activate the type I OSM receptor while only OSM can activate the type II OSM receptor -
Isoforms & Post-Translational Modification
P40189 has 3 isomers: P40189-1: 103537 Da (predicted); P40189-2: 37499 Da (predicted); P40189-3: 96276 Da (predicted).
Phosphorylation of Ser-782 down-regulates cell surface expression;Heavily N-glycosylated (PubMed:11098061, PubMed:11251120, PubMed:16335952, PubMed:19139490, PubMed:19159218). Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding (PubMed:19915009) -
Subunit
Component of a hexamer of two molecules each of IL6, IL6R and IL6ST; associates with the complex IL6:IL6R but does not interact with IL6 (PubMed:12829785, PubMed:2261637). Forms heterodimers composed of LIFR and IL6ST (type I OSM receptor) which are activated by LIF and OSM (PubMed:8999038). Also forms heterodimers composed of OSMR and IL6ST (type II receptor) which are activated by OSM but not by LIF (PubMed:8999038). Component of a receptor complex composed of IL6ST/GP130, IL27RA/WSX1 and CNTFR which interacts with the neuroprotective peptide humanin (PubMed:19386761). Interacts with HCK (PubMed:9406996). Interacts with INPP5D/SHIP1 (By similarity). Interacts with SRC and YES (PubMed:25731159). Interacts with ARMH4; this interaction prevents IL6ST protein homodimerization and bridges ARMH4 with IL6R and STAT3 and therefore inhibits phosphorylation of STAT3 at 'Tyr-705' (PubMed:26927669)
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SwissProt ID
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Synonyms
P2Y1
Documentation
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