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NCF1 Antibody (YA2575)

(Synonyms: NCF1; NCF1A; NOXO2; SH3PXD1A; p47phox)
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Based on 1 Customer Validation

NCF1 Antibody (YA2575) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • NCF1 Antibody (YA2575) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

NCF1 Antibody (YA2575) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database

Entrez Gene: 653361 Human

SwissProt: P14598 Human

Immunogen

A synthesized peptide derived from human NCF1 aa270-390/390.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104924

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NCF1 Antibody (YA2575)
    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), and Daudi (lane 3, 15 μg) using NCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NCF1 Antibody (YA2575)
    Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using NCF1 Antibody (HY-P82830) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    NCF1 is a Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytosol; Membrane; Peripheral membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:Detected in peripheral blood monocytes and neutrophils (at protein level)

  • Isoforms & Post-Translational Modification

    P14598 has 2 isomers: P14598-1: 44682 Da (predicted); P14598-2: 30811 Da (predicted).
    Phosphorylated by PRKCD; phosphorylation induces activation of NCF1, leading to assembly and activation of the NADPH oxidase complex

  • Subunit

    Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:38355798). Part of a cytosolic complex composed at least by NCF1, NCF2 and NCF4 (PubMed:8280052). Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain) (PubMed:12169629). Interacts with NCF4 (PubMed:15657040). Interacts with CYBB (PubMed:9224653). Interacts (via the second SH3 domain) with CYBA; interaction is phosphorylation-dependent (PubMed:12732142, PubMed:16326715). Interacts with NOXA1 (PubMed:12716910). Interacts with ADAM15 (PubMed:19718658). Interacts with TRAF4 (PubMed:12023963, PubMed:16052631). Interacts with FASLG (PubMed:19807924). Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity)

  • SwissProt ID

    P14598

  • Gene ID
    653361 [NCBI]
  • Synonyms

    NCF1; NCF1A; NOXO2; SH3PXD1A; p47phox

  • Research Field

    -

NCF1 Antibody (YA2575) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), and Daudi (lane 3, 15 μg) using NCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using NCF1 Antibody (HY-P82830) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NCF1 Antibody (YA2575) Product Image
    1/16
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • NCF1 Antibody (YA2575) Product Image
  • Experimental Validation Results for NCF1 Antibody (YA2575)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), and Daudi (lane 3, 15 μg) using NCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NCF1 Antibody (YA2575)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using NCF1 Antibody (HY-P82830) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • Experimental Validation Results for NCF1 Antibody (YA2575)
  • NCF1 Antibody (YA2575) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), and Daudi (lane 3, 15 μg) using NCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • NCF1 Antibody (YA2575) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using NCF1 Antibody (HY-P82830) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NCF1 Antibody (YA2575) Product Image WB
  • NCF1 Antibody (YA2575) Product Image WB