NCF1 Antibody (YA2575)

Cat. No.: HY-P82830: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

NCF1 Antibody (YA2575) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCF1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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NCF1 Antibody (YA2575) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

NCF1 Antibody (YA2575) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCF1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P14598

Gene ID

653361 [NCBI]

Immunogen

A synthesized peptide derived from human NCF1 aa270-390/390.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), Ramos (lane 2, 15 μg), and Daudi (lane 3, 15 μg) using NCF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using NCF1 Antibody (HY-P82830) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:2547247, PubMed:2550933, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed:12732142, PubMed:19801500)

Subcellular Localization：Cytoplasm, cytosol; Membrane; Peripheral membrane protein; Cytoplasmic side

Expression：
Tissue_specificity:Detected in peripheral blood monocytes and neutrophils (at protein level)

Isoforms & Post-Translational Modification：P14598 has 2 isomers: P14598-1: 44682 Da (predicted); P14598-2: 30811 Da (predicted).
Phosphorylated by PRKCD; phosphorylation induces activation of NCF1, leading to assembly and activation of the NADPH oxidase complex

Subunit：Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:38355798). Part of a cytosolic complex composed at least by NCF1, NCF2 and NCF4 (PubMed:8280052). Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain) (PubMed:12169629). Interacts with NCF4 (PubMed:15657040). Interacts with CYBB (PubMed:9224653). Interacts (via the second SH3 domain) with CYBA; interaction is phosphorylation-dependent (PubMed:12732142, PubMed:16326715). Interacts with NOXA1 (PubMed:12716910). Interacts with ADAM15 (PubMed:19718658). Interacts with TRAF4 (PubMed:12023963, PubMed:16052631). Interacts with FASLG (PubMed:19807924). Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity)

RRID

AB_3104924

Database

Entrez Gene: 653361 Human

SwissProt: P14598 Human

OMIM: 233700 Human

Research Field

Synonyms

NCF1; NCF1A; NOXO2; SH3PXD1A; p47phox

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

NCF1 Antibody (YA2575) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NCF1 Antibody (YA2575)

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Background

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NCF1 Antibody (YA2575) Related Classifications

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