Phospho-MYL9(Thr18/Ser19) Antibody (YA5774)

Cat. No.: HY-P86082: Data Sheet COA
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Phospho-MYL9(Thr18/Ser19) Antibody (YA5774) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MYL9(Thr18/Ser19).

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

Phospho-MYL9(Thr18/Ser19) Antibody (YA5774) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MYL9(Thr18/Ser19).

Host

Rabbit

Clonality

Monoclonal

분자량

Predicted band size: 20 kDa;

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P24844

Gene ID

10398 [NCBI] / 10627 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:400
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Purity	Protein A	Conjugation	Non-conjugated
Modification	Phosphorylated	Isotype	IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cell cortex

Expression：
Tissue_specificity:Smooth muscle tissue and some (but not all) non-muscle cells

Isoforms & Post-Translational Modification：P24844 has 2 isomers: P24844-1: 19827 Da (predicted); P24844-2: 13866 Da (predicted).
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation. Phosphorylated by DAPK3; DAPK3-mediated phosphorylation is inhibited by LUZP1 (PubMed:38009294)

Subunit：Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19 (By similarity). Interacts with LUZP1; the interaction results in inhibition of phosphorylation of MYL9 by DAPK3 (PubMed:38009294)

Synonyms

MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL

각종 서류

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Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)