VCAM1 Antibody (YA017)(PBS only)

Cat. No.: HY-P80927A: Data Sheet User Guide for Antibodies
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VCAM1 Antibody (YA017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VCAM1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

VCAM1 Antibody (YA017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VCAM1.

Host

Rabbit

분자량

Predicted band size: 81 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P19320

Gene ID

7412 [NCBI]

Immunogen

Synthetic peptide corresponding to VCAM1 C-term.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:It is expressed on inflammatory vascular endothelial cells, as well as macrophage-like cells and dendritic cells in normal and inflammatory tissues.

Induction:By pro-inflammatory cytokines, including TNFalpha, and also by ROS, oxidized low density lipoprotein, high glucose concentration, toll-like receptor agonists, and shear stress

Isoforms & Post-Translational Modification：P19320 has 3 isomers: P19320-1: 81276 Da (predicted); P19320-2: 71255 Da (predicted); P19320-3: 74346 Da (predicted).
Cleaved by the metalloproteinase ADAM17 to generate the soluble form;Sialoglycoprotein;Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation

Research Field

Signal Transduction

Synonyms

VCAM1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD antigen CD106

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h1650-cell.html