Copy Link Print Information

ABCG1 Antibody (YA1841)

(Synonyms: ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1; ABCG1; ABC transporter 8; WHITE1)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ABCG1 Antibody (YA1841)
  • ABCG1 Antibody (YA1841) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) ABCG1 Antibody (YA1841)

More

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 76 kDa
Species Reactivity Database

Entrez Gene: 9619 Human

SwissProt: P45844 Human

Immunogen

A synthetic peptide of human ABCG1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104212

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ABCG1 Antibody (YA1841)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using ABCG1 Antibody (HY-P82096) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ABCG1 catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol[1][2][3].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cell membrane

  • Expression


    Tissue_specificity:Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from with Tangier disease

    Induction:Strongly induced in monocyte-derived macrophages during cholesterol influx. Conversely, mRNA and protein expression are suppressed by lipid efflux. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Not induced by bacterial lipopolysaccharides (LPS) . Repressed by ZNF202

  • Isoforms & Post-Translational Modification

    P45844 has 8 isomers: P45844-1: 75592 Da (predicted); P45844-2: 75380 Da (predicted); P45844-3: 74611 Da (predicted); P45844-4: 74155 Da (predicted); P45844-5: 74032 Da (predicted); P45844-6: 73737 Da (predicted); P45844-7: 71960 Da (predicted); P45844-8: 86628 Da (predicted).
    Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum

  • Subunit

    Homodimer; disulfide-linked (PubMed:16702602). Homooligomer (PubMed:16702602). May form heterodimers with several heterologous partners of the ABCG subfamily. Forms heterodimers with ABCG4 (PubMed:27228027). Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux (PubMed:24576892)

  • SwissProt ID

    P45844

  • Gene ID
    9619 [NCBI]
  • Synonyms

    ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1; ABCG1; ABC transporter 8; WHITE1

  • Research Field

    Neuroscience

ABCG1 Antibody (YA1841) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using ABCG1 Antibody (HY-P82096) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ABCG1 Antibody (YA1841) Product Image
    1/16
  • Experimental Validation Results for ABCG1 Antibody (YA1841)
  • ABCG1 Antibody (YA1841) Product Image
  • Experimental Validation Results for ABCG1 Antibody (YA1841)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using ABCG1 Antibody (HY-P82096) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCG1 Antibody (YA1841)
  • ABCG1 Antibody (YA1841) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using ABCG1 Antibody (HY-P82096) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ABCG1 Antibody (YA1841) Product Image WB