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STAT5B Antibody (YA5880)

(Synonyms: Signal transducer and activator of transcription 5B)
Customer Review

Based on 1 Customer Validation

STAT5B Antibody (YA5880) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for STAT5B Antibody (YA5880)
  • STAT5B Antibody (YA5880) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

STAT5B Antibody (YA5880) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5B.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 90 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STAT5B Antibody (YA5880)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using STAT5B Antibody (HY-P86188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    STAT5B is a Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation[1].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Subunit

    Upon activation, forms homodimers (PubMed:29844444).

  • SwissProt ID

    P51692

  • Gene ID
    6777 [NCBI]
  • Synonyms

    Signal transducer and activator of transcription 5B

STAT5B Antibody (YA5880) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using STAT5B Antibody (HY-P86188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • STAT5B Antibody (YA5880) Product Image
    1/16
  • Experimental Validation Results for STAT5B Antibody (YA5880)
  • STAT5B Antibody (YA5880) Product Image
  • Experimental Validation Results for STAT5B Antibody (YA5880)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using STAT5B Antibody (HY-P86188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STAT5B Antibody (YA5880)
  • STAT5B Antibody (YA5880) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using STAT5B Antibody (HY-P86188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • STAT5B Antibody (YA5880) Product Image WB