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Phospho-FAK (Tyr397) Antibody (YA7376)

(Synonyms: FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71)
Customer Review

Based on 1 Customer Validation

Phospho-FAK (Tyr397) Antibody (YA7376) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-FAK (Tyr397).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Phospho-FAK (Tyr397) Antibody (YA7376) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-FAK (Tyr397).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 119 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 119 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
    HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with Calyculin A (HY-18983) at concentration of 10 nM for 30 mins. Phospho-FAK (Tyr397) protein expression was subsequently analyzed by Western blot. The results demonstrated that Calyculin A induced phosphorylation of FAK (Tyr397). Primary antibodies used: Phospho-FAK (Tyr397) Antibody (HY-P87691) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
    Western blot analysis of extracts from Hela (lane 2, 20 μg), Hela (lane 3, 20 μg, Calyculin A (HY-18983), 10 nM, 30 mins) as indicated using Phospho-FAK (Tyr397) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1 hour at room temperature. The primary antibody (1:1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/5000) was used for 1 hour at room temperature.

Background

  • Function

    FAK (Focal Adhesion Kinase or PTK2) is a non-receptor and non-membrane associated protein tyrosine kinase that is activated at the sites of cell-matrix adhesions and integrin clustering by auto-phosphorylation (at Tyr397), Src, and other tyrosine kinases. FAK mediates integrin-based cell signaling by transferring signals regulating cell migration, adhesion, and survival from the extracellular matrix to the cytoplasm. FAK is overexpressed in many tumors, including those derived from the head and neck, colon, breast, prostate, liver, and thyroid. Furthermore, FAK overexpression is highly correlated with an invasive phenotype in these tumors. Inhibition of FAK signaling by overexpression of dominant-negative fragments of FAK reduces invasion of glioblastomas and ovarian cancer cells. FAK therefore represents an important target for the development of anti-neoplastic and anti-metastatic drugs.

  • Subcellular Localization

    Cell junction, focal adhesion,Cell membrane,Cytoplasm, perinuclear region,Cytoplasm, cell cortex,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Nucleus,Cytoplasm, cytoskeleton, cilium basal body,Cytoplasm

  • Expression


    Tissue_Specificity: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787)

  • Isoforms & Post-Translational Modification

    Q05397 has 7 isomers: Q05397-1: 119233 Da (predicted); Q05397-2: 99358 Da (predicted); Q05397-3: 63201 Da (predicted); Q05397-4: 48967 Da (predicted); Q05397-5: 120900 Da (predicted); Q05397-6: 39874 Da (predicted); Q05397-7: 114253 Da (predicted).
    Phosphorylated on tyrosine residues upon activation, e丨Sumoylated; this enhances autophosphorylation

  • Subunit

    Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, and CASS4

  • SwissProt ID

    Q05397

  • Synonyms

    FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

Phospho-FAK (Tyr397) Antibody (YA7376) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with Calyculin A (HY-18983) at concentration of 10 nM for 30 mins. Phospho-FAK (Tyr397) protein expression was subsequently analyzed by Western blot. The results demonstrated that Calyculin A induced phosphorylation of FAK (Tyr397). Primary antibodies used: Phospho-FAK (Tyr397) Antibody (HY-P87691) and GAPDH Antibody (HY-P80954A).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20 μg), Hela (lane 3, 20 μg, Calyculin A (HY-18983), 10 nM, 30 mins) as indicated using Phospho-FAK (Tyr397) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1 hour at room temperature. The primary antibody (1:1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/5000) was used for 1 hour at room temperature.

  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image
    1/16
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
    1/1
    MedChemExpress Validation
     HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with Calyculin A (HY-18983) at concentration of 10 nM for 30 mins. Phospho-FAK (Tyr397) protein expression was subsequently analyzed by Western blot. The results demonstrated that Calyculin A induced phosphorylation of FAK (Tyr397). Primary antibodies used: Phospho-FAK (Tyr397) Antibody (HY-P87691) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20 μg), Hela (lane 3, 20 μg, Calyculin A (HY-18983), 10 nM, 30 mins) as indicated using Phospho-FAK (Tyr397) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1 hour at room temperature. The primary antibody (1:1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/5000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Experimental Validation Results for Phospho-FAK (Tyr397) Antibody (YA7376)
  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image
    MedChemExpress Validation

    HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with Calyculin A (HY-18983) at concentration of 10 nM for 30 mins. Phospho-FAK (Tyr397) protein expression was subsequently analyzed by Western blot. The results demonstrated that Calyculin A induced phosphorylation of FAK (Tyr397). Primary antibodies used: Phospho-FAK (Tyr397) Antibody (HY-P87691) and GAPDH Antibody (HY-P80954A).

  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20 μg), Hela (lane 3, 20 μg, Calyculin A (HY-18983), 10 nM, 30 mins) as indicated using Phospho-FAK (Tyr397) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1 hour at room temperature. The primary antibody (1:1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/5000) was used for 1 hour at room temperature.

  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image WB
  • Phospho-FAK (Tyr397) Antibody (YA7376) Product Image WB