YTHDC2 Antibody (YA3317)(PBS only)

Cat. No.: HY-P83572A: Data Sheet User Guide for Antibodies
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YTHDC2 Antibody (YA3317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDC2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

YTHDC2 Antibody (YA3317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDC2.

Host

Rabbit

Molecular Weight

Predicted band size: 161 kDa;

Observed band size: 161 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

B2RR83

Gene ID

240255 [NCBI]

Immunogen

Recombinant protein of mouse YTHDC2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells (PubMed:28380054, PubMed:28809393, PubMed:29033321, PubMed:29087293, PubMed:29360036, PubMed:32470506). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability (PubMed:29360036). Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (PubMed:29033321). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease (PubMed:29033321). Required for both spermatogenesis and oogenesis (PubMed:28809393, PubMed:29033321)

Subcellular Localization：Cytoplasm; Cytoplasm, perinuclear region

Expression：
Tissue_specificity:Present in male and female germ cells (at protein level) (PubMed:29033321, PubMed:32470506) . Highly expressed in testis (PubMed:28809393, PubMed:29033321, PubMed:29087293, PubMed:29360036) . Not detected in spermatogonia next to the tubule wall but is strongly expressed in spermatocytes, suggesting that it is up-regulated in germ cells upon entry into meiosis (at protein level) (PubMed:29087293)

Subunit：Interacts with MEIOC; binds transcripts that regulate the mitotic cell cycle inhibiting progression into metaphase, thereby allowing meiotic prophase to proceed normally (PubMed:28380054, PubMed:29087293).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

CAHL; hYTHDC2

Documentation

User Guide for Antibodies (1077 KB)