ACHE Antibody (YA3883)(PBS only)

Cat. No.: HY-P84186A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

ACHE Antibody (YA3883) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACHE.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ACHE Antibody (YA3883) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACHE.

Host

Mouse

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey, Rat

SwissProt ID

P22303

Gene ID

43 [NCBI]

Immunogen

Purified recombinant fragment of human ACHE aa 587-611(KLH).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis

Subcellular Localization：Synapse; Secreted; Cell membrane; Peripheral membrane protein; Nucleus; Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side

Expression：
Tissue_specificity:Isoform H is highly expressed in erythrocytes

Isoforms & Post-Translational Modification：P22303 has 4 isomers: P22303-1: 67796 Da (predicted); P22303-2: 67376 Da (predicted); P22303-4: 65560 Da (predicted); P22303-3: 58352 Da (predicted).

Subunit：Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity). Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric

Synonyms

YT; ACEE; ARACHE; N-ACHE

Documentation

User Guide for Antibodies (1077 KB)