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STING Antibody (YA6309)

(Synonyms: TMEM173; ERIS; MITA; STING; Transmembrane protein 173; Endoplasmic reticulum interferon stimulator; ERIS; Mediator of IRF3 activation; hMITA; Stimulator of interferon genes protein; hSTING)
Customer Review

Based on 1 Customer Validation

STING Antibody (YA6309) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STING.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • STING Antibody (YA6309) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1000 1:1000-5000 1:200-1000 1:5000-20000 1:50-200

Product Details

Description

STING Antibody (YA6309) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STING.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719191

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STING Antibody (YA6309)
    Western blot analysis of extracts from THP-1 (lane 2(20μg), HT-29 (lane 3(20μg), HL-60 (lane 4(20μg) and K562 (lane 5(20μg) using STING Antibody (HY-P86617) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    Immunohistochemical analysis of paraffin-embedded human bone marrowl tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Stimulator of interferon genes (STING) is an integral ER-membrane protein that can be activated by 2'3'-cGAMP synthesized by cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) upon binding of double-stranded DNA. It activates interferon (IFN) and inflammatory cytokine responses to defend against infection by microorganisms. STING is a key cytosolic receptor for small nucleotides and plays a key role in anticancer and antiviral immunity. STING signaling pathway is also a critical link between innate and adaptive immunity, and induces anti-tumor immune responses. STING agonists, such as endogenous cyclic dinucleotide (CDN) cyclic GMP-AMP (cGAMP), have been used in diverse research for immunogenic tumor clearance, antiviral treatments and vaccine adjuvants.

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region; Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein; Mitochondrion outer membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed (PubMed:18724357, PubMed:18818105) . Expressed in skin endothelial cells, type II alveolar cells, bronchial epithelial cells, and alveolar macrophages (PubMed:25029335) .

  • Subunit

    Homodimer; forms a homodimer in absence of cyclic nucleotide (c-di-GMP or cGAMP); 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization (PubMed:19285439, PubMed:22579474, PubMed:22705373, PubMed:22728658, PubMed:22728659, PubMed:22728660, PubMed:30842659). Homotetramer; in presence of cyclic nucleotide (c-di-GMP or cGAMP), forms tetramers and higher-order oligomers through side-by-side packing (PubMed:30842653, PubMed:35388221). Interacts (when phosphorylated) with IRF3; following activation and phosphorylation on the pLxIS motif by TBK1, recruits IRF3 (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:28331227). Interacts with RIGI, MAVS and SSR2 (PubMed:18724357, PubMed:18818105). Interacts with RNF5 and TRIM56 (PubMed:19285439, PubMed:21074459). Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta (PubMed:19416887). Interacts with IFIT1 and IFIT2 (PubMed:19416887). Interacts with TRIM29; this interaction induces STING1 ubiquitination and subsequent degradation (PubMed:29038422). Associates with the MHC-II complex (By similarity). Interacts with STEEP1; interaction takes place upon cGAMP-activation and STING1 phosphorylation by MAP3K7/TAK1 and promotes STING1 translocation to COPII vesicles (PubMed:32690950, PubMed:37832545). Interacts with SEC24A, SEC24B, and SEC24C; promoting translocation to COPII vesicles (PubMed:30842662, PubMed:32690950). Interacts (when ubiquitinated) with SQSTM1; leading to relocalization to autophagosomes (By similarity). Interacts with SURF4 (PubMed:29251827). Interacts with HNRNPA2B1 (PubMed:31320558). Interacts with ZDHHC1; ZDHHC1 constitutively interacts with STING1 and in presence of DNA viruses activates it by promoting its cGAMP-induced oligomerization and the recruitment of downstream signaling components (PubMed:25299331). Interacts with ZDHHC11; in presence of DNA viruses promotes the recruitment of IRF3 to STING1 (PubMed:28331227). Interacts with TOMM70 (PubMed:20628368). Interacts with isoform IFI16-beta of IFI16 (PubMed:30104205). Interacts with TAB1; promoting recruitment of TAB1 to the endoplasmic reticulum membrane and subsequent activation of MAP3K7/TAK1 (PubMed:37832545). Interacts (via transmembrane domain) with TMEM203 (By similarity). Interacts with DDX41 (PubMed:25704810). Interacts with TMEM120A (via C-terminal domain); regulates the trafficking of STING1 from the ER to the ER-Golgi intermediate compartment to elicit antiviral effects (PubMed:35013224)

  • SwissProt ID

    Q86WV6

  • Gene ID
    340061 [NCBI]
  • Synonyms

    TMEM173; ERIS; MITA; STING; Transmembrane protein 173; Endoplasmic reticulum interferon stimulator; ERIS; Mediator of IRF3 activation; hMITA; Stimulator of interferon genes protein; hSTING

STING Antibody (YA6309) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), HT-29 (lane 3(20μg), HL-60 (lane 4(20μg) and K562 (lane 5(20μg) using STING Antibody (HY-P86617) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrowl tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    1/16
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • STING Antibody (YA6309) Product Image
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), HT-29 (lane 3(20μg), HL-60 (lane 4(20μg) and K562 (lane 5(20μg) using STING Antibody (HY-P86617) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrowl tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • Experimental Validation Results for STING Antibody (YA6309)
  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2(20μg), HT-29 (lane 3(20μg), HL-60 (lane 4(20μg) and K562 (lane 5(20μg) using STING Antibody (HY-P86617) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bone marrowl tissue using STING Antibody (HY-P86617, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • STING Antibody (YA6309) Product Image WB
  • STING Antibody (YA6309) Product Image IHC-P
  • STING Antibody (YA6309) Product Image IHC-P
  • STING Antibody (YA6309) Product Image IHC-P
  • STING Antibody (YA6309) Product Image IHC-P
  • STING Antibody (YA6309) Product Image IHC-P
  • STING Antibody (YA6309) Product Image IHC-P