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CD168 Antibody (YA1322)

(Synonyms: IHABP, RHAMM, HMMR)
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Based on 1 Customer Validation

CD168 Antibody (YA1322) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD168.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD168 Antibody (YA1322)
  • CD168 Antibody (YA1322) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:1000 1:1000

Product Details

Description

CD168 Antibody (YA1322) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD168.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size:84 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Species Reactivity Database
Immunogen

Recombinant protein within human CD168 aa 1-350 / 724 (O75330).

Sensitivity

Endogenous

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD168 Antibody (YA1322)
    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CD168 receptor for hyaluronic acid (HA). Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis

  • Subcellular Localization

    Cell surface; Cytoplasm; Cytoplasm, cytoskeleton, spindle

  • Expression


    Tissue_specificity:It is expressed in the testes (PubMed: 22965910) . It is expressed in the mammary gland (PubMed: 8890751) .

  • Isoforms & Post-Translational Modification

    O75330 has 4 isomers: O75330-1: 84100 Da (predicted); O75330-2: 82301 Da (predicted); O75330-3: 84229 Da (predicted); O75330-4: 74495 Da (predicted).

  • Subunit

    Interacts with ANKRD26 (PubMed:22666460). Interacts with DYNLL1 (PubMed:22965910). Interacts with FAM83D/CHICA (PubMed:22965910)

  • SwissProt ID

    O75330

  • Gene ID
    3161 [NCBI]
  • Synonyms

    IHABP, RHAMM, HMMR

  • Research Field

    Cell biology

CD168 Antibody (YA1322) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD168 Antibody (YA1322) Product Image
    1/16
  • Experimental Validation Results for CD168 Antibody (YA1322)
  • CD168 Antibody (YA1322) Product Image
  • Experimental Validation Results for CD168 Antibody (YA1322)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD168 Antibody (YA1322)
  • CD168 Antibody (YA1322) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD168 Antibody (YA1322) Product Image WB