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PLGF Antibody (YA1812)

(Synonyms: Pgf; PGFL; PIGF; Placenta growth factor; Placental growth factor; vascular endothelial growth factor related protein; PlGF; PlGF2; SHGC 10760)
Customer Review

Based on 1 Customer Validation

PLGF Antibody (YA1812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLGF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • PLGF Antibody (YA1812) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200

Product Details

Description

PLGF Antibody (YA1812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLGF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human PLGF aa 48-97 / 221.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104022

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PLGF Antibody (YA1812)
    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using PLGF Antibody (HY-P82067). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLGF Antibody (YA1812)
    Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PLGF is a Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Although all three isoforms are present in most placental tissues, PlGF-2 is specific to the early (8-week) placenta, while only PlGF-1 is found in colon and breast cancer.

  • Isoforms & Post-Translational Modification

    P49763 has 4 isomers: P49763-1: 24789 Da (predicted); P49763-2: 16725 Da (predicted); P49763-3: 19338 Da (predicted); P49763-4: 27402 Da (predicted).
    N-glycosylated

  • Subunit

    Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF. Isoform PlGF-3 is found both as homodimer and as monomer

  • SwissProt ID

    P49763

  • Gene ID
    5228 [NCBI]
  • Synonyms

    Pgf; PGFL; PIGF; Placenta growth factor; Placental growth factor; vascular endothelial growth factor related protein; PlGF; PlGF2; SHGC 10760

  • Research Field

    Cardiovascular

PLGF Antibody (YA1812) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using PLGF Antibody (HY-P82067). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLGF Antibody (YA1812) Product Image
    1/16
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • PLGF Antibody (YA1812) Product Image
  • Experimental Validation Results for PLGF Antibody (YA1812)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using PLGF Antibody (HY-P82067). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PLGF Antibody (YA1812)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLGF Antibody (YA1812)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • Experimental Validation Results for PLGF Antibody (YA1812)
  • PLGF Antibody (YA1812) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and Hela (lane 5, 20ug) using PLGF Antibody (HY-P82067). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PLGF Antibody (YA1812) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PLGF Antibody (YA1812) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PLGF Antibody (YA1812). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82067, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PLGF Antibody (YA1812) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLGF Antibody (YA1812) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling PLGF with PLGF Antibody (HY-P82067) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PLGF Antibody (HY-P82067) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLGF Antibody (YA1812) Product Image WB
  • PLGF Antibody (YA1812) Product Image IHC-P
  • PLGF Antibody (YA1812) Product Image IHC-P
  • PLGF Antibody (YA1812) Product Image ICC
  • PLGF Antibody (YA1812) Product Image ICC