Thrombospondin-5 Antibody
(Synonyms: Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5)Thrombospondin-5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Thrombospondin-5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Thrombospondin-5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Thrombospondin-5.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 77; 82 kDa
Purified recombinant protein of human Thrombospondin-5
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Thrombospondin-5 plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity)[1][2][3].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix
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Expression
Tissue_Specificity: Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect. -
Isoforms & Post-Translational Modification
Thrombospondin-5 has 2 isoforms, P49747-1: amino acid length is 757, molecular weight is 82860 Da (predicted); P49747-2: amino acid length is 704, molecular weight is 77214 Da (predicted).
Proteolytically cleaved by metalloproteases ADAMTS4 and ADAMTS1 with ADAMTS4 showing more potent activity. -
Subunit
Pentamer; disulfide-linked.
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SwissProt ID
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Synonyms
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5
Documentation
[1]. Chen FH, et al. Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins. J Biol Chem. 2005 Sep 23;280(38):32655-61. [Content Brief]
[2]. Koelling S, et al. Cartilage oligomeric matrix protein is involved in human limb development and in the pathogenesis of osteoarthritis. Arthritis Res Ther. 2006;8(3):R56. [Content Brief]
[3]. Gagarina V, et al. Cartilage oligomeric matrix protein protects cells against death by elevating members of the IAP family of survival proteins. J Biol Chem. 2008 Jan 4;283(1):648-659. [Content Brief]
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