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HSD3B1 Antibody (YA3519)

(Synonyms: 3BHS1_HUMAN; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase 1; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase 1; EC:1.1.1.145; 3-beta-hydroxysteroid 3-dehydrogenase 1; EC:1.1.1.270; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase. EC:1.1.1.210; Steroid Delta-isomerase 1; EC:5.3.3.1; Trophoblast antigen FDO161G; 3BH; HSDB3A; )
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Based on 1 publication(s) in Google Scholar

HSD3B1 Antibody (YA3519) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HSD3B1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • HSD3B1 Antibody (YA3519) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

HSD3B1 Antibody (YA3519) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HSD3B1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 3283 Human

SwissProt: P14060 Human

Immunogen

A synthesized peptide derived from human HSD3B1 aa300-325.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103268

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    Western blot analysis of extracts from MCF-7(lane 2, 20μg), U937(lane 3, 20μg), SiHa(lane 4, 20μg) and Jurkat(lane 5, 20μg) using HSD3B1 Antibody (HY-P81196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    Immunocytochemistry analysis of HeLa cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    Immunocytochemistry analysis of MCF-7 cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    HSD3B1 is an A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable)[1][2].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass membrane protein; Mitochondrion membrane; Single-pass membrane protein

  • Expression


    Tissue_specificity:Placenta and skin (PubMed:1401999) . Predominantly expressed in mammary gland tissue

  • SwissProt ID

    P14060

  • Gene ID
    3283 [NCBI]
  • Synonyms

    3BHS1_HUMAN; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase 1; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase 1; EC:1.1.1.145; 3-beta-hydroxysteroid 3-dehydrogenase 1; EC:1.1.1.270; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase. EC:1.1.1.210; Steroid Delta-isomerase 1; EC:5.3.3.1; Trophoblast antigen FDO161G; 3BH; HSDB3A;

  • Research Field

    Signal Transduction

[1]. Dumont M, et al. Characterization, expression, and immunohistochemical localization of 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase in human skin. J Invest Dermatol. 1992 Oct;99(4):415-21. [Content Brief]

[2]. Lorence MC, et al. Human 3 beta-hydroxysteroid dehydrogenase/delta 5----4isomerase from placenta: expression in nonsteroidogenic cells of a protein that catalyzes the dehydrogenation/isomerization of C21 and C19 steroids. Endocrinology. 1990 May;126(5):2493-8. [Content Brief]

HSD3B1 Antibody (YA3519) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2, 20μg), U937(lane 3, 20μg), SiHa(lane 4, 20μg) and Jurkat(lane 5, 20μg) using HSD3B1 Antibody (HY-P81196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HSD3B1 Antibody (YA3519) Product Image
    1/16
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • HSD3B1 Antibody (YA3519) Product Image
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2, 20μg), U937(lane 3, 20μg), SiHa(lane 4, 20μg) and Jurkat(lane 5, 20μg) using HSD3B1 Antibody (HY-P81196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • Experimental Validation Results for HSD3B1 Antibody (YA3519)
  • HSD3B1 Antibody (YA3519) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2, 20μg), U937(lane 3, 20μg), SiHa(lane 4, 20μg) and Jurkat(lane 5, 20μg) using HSD3B1 Antibody (HY-P81196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • HSD3B1 Antibody (YA3519) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HSD3B1 Antibody (YA3519) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling HSD3B1 with HSD3B1 antibody (HY-P81196) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSD3B1 antibody (HY-P81196) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HSD3B1 Antibody (YA3519) Product Image WB
  • HSD3B1 Antibody (YA3519) Product Image ICC
  • HSD3B1 Antibody (YA3519) Product Image ICC